Modomics - A Database of RNA Modifications

ID Card:

Full name: La ribonucleoprotein 7, transcriptional regulator
Synonym: HDCMA18P,PIP7S,DKFZP564K112
GI: 121945944
UniProt: Q4G0J3
Structures: | 4WKR | 5KNW | 6D12 | 7SLP | 7SLQ |
Alpha Fold Predicted Structure: AF-Q4G0J3-F1


PDB Structures:


4WKR

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

The non-coding RNA 7SK is the scaffold for a small nuclear ribonucleoprotein (7SKsnRNP) which regulates the function of the positive transcription elongation factor P-TEFb in the control of RNA polymerase II elongation in metazoans. The La-related protein LARP7 is a component of the 7SKsnRNP required for stability and function of the RNA. To address the function of LARP7 we determined the crystal structure of its La module, which binds a stretch of uridines at the 3'-end of 7SK. The structure shows that the penultimate uridine is tethered by the two domains, the La-motif and the RNA-recognition motif (RRM1), and reveals that the RRM1 is significantly smaller and more exposed than in the La protein. Sequence analysis suggests that this impacts interaction with 7SK. Binding assays, footprinting and small-angle scattering experiments show that a second RRM domain located at the C-terminus binds the apical loop of the 3' hairpin of 7SK, while the N-terminal domains bind at its foot. Our results suggest that LARP7 uses both its N- and C-terminal domains to stabilize 7SK in a closed structure, which forms by joining conserved sequences at the 5'-end with the foot of the 3' hairpin and has thus functional implications.

Download RCSB-PDB Structures:

Pdb Files   4WKR.pdb   5KNW.pdb   6D12.pdb   7SLP.pdb   7SLQ.pdb  
Pdbx/mmCIF Files   4WKR.cif   5KNW.cif   6D12.cif   7SLP.cif   7SLQ.cif  


Protein sequence:

METESGNQEKVMEEESTEKKKEVEKKKRSRVKQVLADIAKQVDFWFGDANLHKDRFLREQIEKSRDGYVDISLLVSFNKMKKLTTDGKLIARALRSSAVVELDLEGTRIRRKKPLGERPKDEDERTVYVELLPKNVNHSWIERVFGKCGNVVYISIPHYKSTGDPKGFAFVEFETKEQAAKAIEFLNNPPEEAPRKPGIFPKTVKNKPIPALRVVEEKKKKKKKKGRMKKEDNIQAKEENMDTSNTSISKMKRSRPTSEGSDIESTEPQKQCSKKKKKRDRVEASSLPEVRTGKRKRSSSEDAESLAPRSKVKKIIQKDIIKEASEASKENRDIEISTEEEKDTGDLKDSSLLKTKRKHKKKHKERHKMGEEVIPLRVLSKSEWMDLKKEYLALQKASMASLKKTISQIKSESEMETDSGVPQNTGMKNEKTANREECRTQEKVNATGPQFVSGVIVKIISTEPLPGRKQVRDTLAAISEVLYVDLLEGDTECHARFKTPEDAQAVINAYTEINKKHCWKLEILSGDHEQRYWQKILVDRQAKLNQPREKKRGTEKLITKAEKIRLAKTQQASKHIRFSEYD

Comments:

None





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M E T E S G N Q E K V M E E E S T E K K K E V E K K K R S R V K Q V L A D I A K Q V D F W F G D A N L H K D R F L R E Q I E K S R D G Y V D I S L L V S F N K M K K L T T D G K L I A R A L R S S A V V E L D L E G T R I R R K K P L G E R P K D E D E R T V Y V E L L P K N V N H S W I E R V F G K C G N V V Y I S I P H Y K S T G D P K G F A F V E F E T K E Q A A K A I E F L N N P P E E A P R K P G I F P K T V K N K P I P A L R V V E E K K K K K K K K G R M K K E D N I Q A K E E N M D T S N T S I S K M K R S R P T S E G S D I E S T E P Q K Q C S K K K K K R D R V E A S S L P E V R T G K R K R S S S E D A E S L A P R S K V K K I I Q K D I I K E A S E A S K E N R D I E I S T E E E K D T G D L K D S S L L K T K R K H K K K H K E R H K M G E E V I P L R V L S K S E W M D L K K E Y L A L Q K A S M A S L K K T I S Q I K S E S E M E T D S G V P Q N T G M K N E K T A N R E E C R T Q E K V N A T G P Q F V S G V I V K I I S T E P L P G R K Q V R D T L A A I S E V L Y V D L L E G D T E C H A R F K T P E D A Q A V I N A Y T E I N K K H C W K L E I L S G D H E Q R Y W Q K I L V D R Q A K L N Q P R E K K R G T E K L I T K A E K I R L A K T Q Q A S K H I R F S E Y D

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q4G0J3-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q4G0J3-F1.cif  
DSSP Secondary Structures   Q4G0J3.dssp  





Diseases connected to this enzyme:

Description Reaction Disease Name
2'-O-methylation of the U6 snRNA is impaired; it might influence RNA structure formation and thus splicing fidelity. N:Nm
Alazami syndrome

Publications:

Title Authors Journal Details PubMed Id DOI