Full name: | tRNA (guanine-N(1)-)-methyltransferase |
---|---|
GI: | 731672 |
Orf: | YHR070W |
COG: | COG2520 |
UniProt: | P38793 |
Alpha Fold Predicted Structure: | AF-P38793-F1 |
Enzyme type: | methyltransferase |
Position of modification - modification: |
t:37 - yW t:37 - m1I t:37 - m1G |
MKIALPVFQKFNRLISSCKMSGVFPYNPPVNRQMRELDRSFFITKIPMCAVKFPEPKNISVFSKNFKNCILRVPRIPHVVKLNSSKPKDELTSVQNKKLKTADGNNTPVTKGVLLHESIHSVEDAYGKLPEDALAFLKENSAEIVPHEYVLDYDFWKAEEILRAVLPEQFLEEVPTGFTITGHIAHLNLRTEFKPFDSLIGQVILDKNNKIECVVDKVSSIATQFRTFPMKVIAGKSDSLVVEQKESNCTFKFDFSKVYWNSRLHTEHERLVKQYFQPGQVVCDVFAGVGPFAVPAGKKDVIVLANDLNPESYKYLKENIALNKVAKTVKSFNMDGADFIRQSPQLLQQWIQDEEGGKITIPLPLKKRHRSQQHNDQQPPQPRTKELIIPSHISHYVMNLPDSAISFLGNFRGIFAAHTKGATDTIQMPWVHVHCFEKYPPGDQVTEDELHARVHARIIAALKVTADDLPLNAVSLHLVRKVAPTKPMYCASFQLPANV
S-Adenosyl methionine (SAM or AdoMet) consists of an adenosyl cation attached to the sulfur methionine. It is synthesized from ATP and methionine by S-Adenosylmethionine synthetase enzyme through the reaction of ATP with L-methionine and water forming as catalytical product S-adenosyl-L-methionine. SAM is a common cosubstrate involved in several reactions, among which methyl group transfers. Trm5 is a site-specific enzyme methylating the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs using SAM as a cosubstrate ( Lee et al. 2007). Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding ( Björk et al. 2001). It also methylates I at position 37.
Alpha Fold Pdb Files | AF-P38793-F1.pdb |
Alpha Fold Pdbx/mmCIF Files | AF-P38793-F1.cif |
DSSP Secondary Structures | P38793.dssp |
_SGD_ |
_PubMed_ |