Modomics - A Database of RNA Modifications

ID Card:

Full name:	tRNA (guanine(26)-N(2))-dimethyltransferase, mitochondrial
GI:	136242
Orf:	YDR120C
COG:	COG1867
UniProt:	P15565
Alpha Fold Predicted Structure:	AF-P15565-F1
Enzyme type:	methyltransferase
Position of modification - modification:	t:26 - m2,2G

Protein sequence:

MEGFFRIPLKRANLHGMLKAAISKIKANFTAYGAPRINIEDFNIVKEGKAEILFPKKETVFYNPIQQFNRDLSVTCIKAWDNLYGEECGQKRNNKKSKKKRCAETNDDSSKRQKMGNGSPKEAVGNSNRNEPYINILEALSATGLRAIRYAHEIPHVREVIANDLLPEAVESIKRNVEYNSVENIVKPNLDDANVLMYRNKATNNKFHVIDLDPYGTVTPFVDAAIQSIEEGGLMLVTCTDLSVLAGNGYPEKCFALYGGANMVSHESTHESALRLVLNLLKQTAAKYKKTVEPLLSLSIDFYVRVFVKVKTSPIEVKNVMSSTMTTYHCSRCGSYHNQPLGRISQREGRNNKTFTKYSVAQGPPVDTKCKFCEGTYHLAGPMYAGPLHNKEFIEEVLRINKEEHRDQDDTYGTRKRIEGMLSLAKNELSDSPFYFSPNHIASVIKLQVPPLKKVVAGLGSLGFECSLTHAQPSSLKTNAPWDAIWYVMQKCDDEKKDLSKMNPNTTGYKILSAMPGWLSGTVKSEYDSKLSFAPNEQSGNIEKLRKLKIVRYQENPTKNWGPKARPNTS

Comments:

S-Adenosyl methionine (SAM or AdoMet) consists of an adenosyl cation attached to the sulfur methionine. It is synthesized from ATP and methionine by S-Adenosylmethionine synthetase enzyme through the the reaction of ATP with L-methionine and water forming as catalytical product S-adenosyl-L-methionine. SAM is a common cosubstrate involved in several reactions, among which methyl group transfers. Trm1 di-methylates a single guanine residue at position 26 of most tRNAs using SAM as a donor of the methyl groups, and is required for the modification of both mitochondrial and cytoplasmic tRNAs.

Search:

Reaction	Substrate	SubstrateType	Position	(Anti)Codon	Modified (Anti)Codon	Transcript Name	Transcript Region	Cellular Localization	References
G:m2G	RNA	tRNA	26	CAC	CAC	tRNA^Val_CAC	~	Mitochondrion and Cytoplasm	2426253
G:m2G	RNA	tRNA	26	UGG	UGG	tRNA^Pro_UGG	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	UGA	UGA	tRNA^Ser_UGA	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	UAG	UAG	tRNA^Thr_UAG	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	GUA	GUA	tRNA^Tyr_GUA	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	IGC	IGC	tRNA^Ala_IGC	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	ICU	ICU	tRNA^Arg_ICU	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	ICG	ICG	tRNA^Arg_ICG	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	GUU	GUU	tRNA^Asn_GUU	~	~
G:m2G	RNA	rRNA	26	UAG	UAG	tRNA^Leu_UAG	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	CAA	CAA	tRNA^Leu_CAA	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	UAA	UAA	tRNA^Leu_UAA	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	CUU	CUU	tRNA^Lys_CUU	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	SUU	SUU	tRNA^Lys_SUU	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	CAU	CAU	tRNA^Met_CAU	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	GAA	GAA	tRNA^Phe_GAA	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	CGA	CGA	tRNA^Ser_CGA	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	UGA	UGA	tRNA^Ser_UGA	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	IGA	IGA	tRNA^Ser_IGA	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	IGU	IGU	tRNA^Thr_IGU	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	GUA	GUA	tRNA^Tyr_GUA	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	CAC	CAC	tRNA^Val_CAC	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	UAA	UAA	tRNA^Leu_UAA	~	Mitochondrion and Cytoplasm
G:m2G	RNA	tRNA	26	GAA	GAA	tRNA^Phe_GAA	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	IGC	IGC	tRNA^Ala_IGC	~	Mitochondrion and Cytoplasm
m2G:m2,2G	tRNA (t)	tRNA	26	ICU	ICU	tRNA^Arg_ICU	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	ICG	ICG	tRNA^Arg_ICG	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	GUU	GUU	tRNA^Asn_GUU	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	UAG	UAG	tRNA^Leu_UAG	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	CAA	CAA	tRNA^Leu_CAA	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	UAA	UAA	tRNA^Leu_UAA	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	CUU	CUU	tRNA^Lys_CUU	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	SUU	SUU	tRNA^Lys_SUU	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	CAU	CAU	tRNA^Met_CAU	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	GAA	GAA	tRNA^Phe_GAA	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	CGA	CGA	tRNA^Ser_CGA	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	UGA	UGA	tRNA^Ser_UGA	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	IGA	IGA	tRNA^Ser_IGA	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	IGU	IGU	tRNA^Thr_IGU	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	GUA	GUA	tRNA^Tyr_GUA	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	CAC	CAC	tRNA^Val_CAC	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	UAA	UAA	tRNA^Leu_UAA	~	Mitochondrion and Cytoplasm
m2G:m2,2G	RNA	tRNA	26	GAA	GAA	tRNA^Phe_GAA	~	Mitochondrion and Cytoplasm


G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
G:m2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G
m2G:m2,2G

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Alpha Fold Predicted Structure:

Downloading... [233210/517076]

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Protein sequence:

M E G F F R I P L K R A N L H G M L K A A I S K I K A N F T A Y G A P R I N I E D F N I V K E G K A E I L F P K K E T V F Y N P I Q Q F N R D L S V T C I K A W D N L Y G E E C G Q K R N N K K S K K K R C A E T N D D S S K R Q K M G N G S P K E A V G N S N R N E P Y I N I L E A L S A T G L R A I R Y A H E I P H V R E V I A N D L L P E A V E S I K R N V E Y N S V E N I V K P N L D D A N V L M Y R N K A T N N K F H V I D L D P Y G T V T P F V D A A I Q S I E E G G L M L V T C T D L S V L A G N G Y P E K C F A L Y G G A N M V S H E S T H E S A L R L V L N L L K Q T A A K Y K K T V E P L L S L S I D F Y V R V F V K V K T S P I E V K N V M S S T M T T Y H C S R C G S Y H N Q P L G R I S Q R E G R N N K T F T K Y S V A Q G P P V D T K C K F C E G T Y H L A G P M Y A G P L H N K E F I E E V L R I N K E E H R D Q D D T Y G T R K R I E G M L S L A K N E L S D S P F Y F S P N H I A S V I K L Q V P P L K K V V A G L G S L G F E C S L T H A Q P S S L K T N A P W D A I W Y V M Q K C D D E K K D L S K M N P N T T G Y K I L S A M P G W L S G T V K S E Y D S K L S F A P N E Q S G N I E K L R K L K I V R Y Q E N P T K N W G P K A R P N T S

Secondary Structure Alphabet

G: 3-turn helix (3₁₀helix)
H: α-helix
I: 𝝅-helix (5 - turn helix)
T: Hydrogen Bonded Turn
B: β-sheet
S: Bend
C: Coil (residues not present in any of the above conformations)
N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files	AF-P15565-F1.pdb
Alpha Fold Pdbx/mmCIF Files	AF-P15565-F1.cif
DSSP Secondary Structures	P15565.dssp

Publications:

Search:

Title	Authors	Journal	Details	PubMed Id	DOI
Isolation and characterization of the TRM1 locus, a gene essential for the N2,N2-dimethylguanosine modification of both mitochondrial and cytoplasmic tRNA in Saccharomyces cerevisiae.	Ellis SR, Morales MJ, Li JM, Hopper AK, Martin NC	J Biol Chem	[details]	2426253	-


Isolation and characterization of the TRM1 locus, a gene essential for the N2,N2-dimethylguanosine modification of both mitochondrial and cytoplasmic tRNA in Saccharomyces cerevisiae.

Showing 1 to 1 of 1 entries