Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA 2-methylthio-N6-isopentenyl adenosine(37) hydroxylase
GI: 312708
Orf: STM4471
COG: COG4445
UniProt: Q08015
Structures: | 2ITB |
Alpha Fold Predicted Structure: AF-Q08015-F1
Enzyme type: hydroxylase
Position of modification - modification: t:37 - ms2io6A


PDB Structures:


2ITB

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:



Download RCSB-PDB Structures:

Pdb Files   2ITB.pdb  
Pdbx/mmCIF Files   2ITB.cif  


Protein sequence:

MTVRQRLLSYFFNRLRMNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICELKAAQTALLLVRKYVADKSGADALLSWLQPYEAFAFRQGPEPDFVALHKQISKSAMPQTDDPWGRQLIDRMVLLIKEELHHFWQVREVMQARNIPYVKITASRYAKGMLKAVRTHEPLTLIDKLICGAYIEARSCERFAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISARVRYFGEVEADLILSPDREFRFHSGVPAAG

Comments:

This iron-containing hydroxylase catalyzes the posttranscriptional allylic hydroxylation of ms2i6A37 in tRNAs. This enzyme is not present in Escherichia coli.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
ms2i6A:ms2io6A RNA tRNA 37 GAA GAA tRNAPheGAA anticodon Prokaryotic Cytosol 9620964   
i6A:io6A RNA tRNA 37 anticdon Prokaryotic Cytosol
ms2i6A:ms2io6A RNA tRNA 37 GCA GCA tRNACysGCA anticodon Prokaryotic Cytosol 9620964   
ms2i6A:ms2io6A RNA tRNA 37 TCA TCA tRNATrpCCA anticodon Prokaryotic Cytosol 9620964   
ms2i6A:ms2io6A RNA tRNA 37 CCA CCA tRNATrpTCA anticodon Prokaryotic Cytosol 9620964   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M T V R Q R L L S Y F F N R L R M N Y P Q I L S P V L N F L H C P T P Q A W I V Q A R D P Q N L P L L L T D H L I C E L K A A Q T A L L L V R K Y V A D K S G A D A L L S W L Q P Y E A F A F R Q G P E P D F V A L H K Q I S K S A M P Q T D D P W G R Q L I D R M V L L I K E E L H H F W Q V R E V M Q A R N I P Y V K I T A S R Y A K G M L K A V R T H E P L T L I D K L I C G A Y I E A R S C E R F A A L A P W L D E D L Q T F Y L S L L R S E A R H Y Q D Y L A L A Q Q I S A E D I S A R V R Y F G E V E A D L I L S P D R E F R F H S G V P A A G

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q08015-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q08015-F1.cif  
DSSP Secondary Structures   Q08015.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
tRNA-modifying MiaE protein from Salmonella typhimurium is a nonheme diiron monooxygenase. Mathevon C, Pierrel F, Oddou JL, Garcia-Serres R, Blondin G, Latour JM, Menage S, Gambarelli S, Fontecave M, Atta M Proc Natl Acad Sci U S A [details] 17679698 -
Structural bioinformatics analysis of enzymes involved in the biosynthesis pathway of the hypermodified nucleoside ms(2)io(6)A37 in tRNA. Kaminska KH, Baraniak U, Boniecki M, Nowaczyk K, Czerwoniec A, Bujnicki JM Proteins [details] 17910062 -
Isolation of the gene (miaE) encoding the hydroxylase involved in the synthesis of 2-methylthio-cis-ribozeatin in tRNA of Salmonella typhimurium and characterization of mutants. Persson BC, Bjork GR J Bacteriol [details] 8253666 -
The ms2io6A37 modification of tRNA in Salmonella typhimurium regulates growth on citric acid cycle intermediates. Persson BC, Olafsson O, Lundgren HK, Hederstedt L, Bjork GR J Bacteriol [details] 9620964 -

Links:

_PubMed_