Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA pseudouridine synthase 4
GI: 1353109
Orf: YNL292W
COG: COG0130
UniProt: P48567
Alpha Fold Predicted Structure: AF-P48567-F1
Enzyme type: pseudouridine synthase
Position of modification - modification: t:55 - Y



Protein sequence:

MNGIFAIEKPSGITSNQFMLKLQHALTKSQVFSKEIQRATAERKQQYEKQTGKKASKRKLRKVSKVKMGHGGTLDPLASGVLVIGIGAGTKKLANYLSGTVKVYESEALFGVSTTSGDVEGEILSQNSVKHLNFDDLKTVEEKFVGQLKQTPPIYAALKMDGKPLHEYAREGKPLPRAIEPRQVTIYDLKVFSDSLKRDHDYPLLRPTTEEAVDTVKNLNANMLNDVLYFSKEYTEKHGLDSEVAKVEEPFPLSEQEEQEIQKEGDSYRAPKLHFKANVSSGTYIRSLVSDIGKSMRSSCYMVKLIRLQQQDWSLEKNNVFQLTDFTERDEKVWSKVLEKVLDEGATVDVIEELKKAEKEIPADVKECIVSSDQPGDEATAETIETANAEEHSNTLKRKIEQV

Comments:

Acts on mitochondrial and cytoplasmic tRNAs. Monomeric enzyme.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
U:Y tRNA (t) Ala/IGC/prokaryotic cytosol 55
U:Y tRNA (t) Arg/ACU/prokaryotic cytosol 55
U:Y tRNA (t) Arg/ICG/prokaryotic cytosol 55
U:Y tRNA (t) Asn/GUU/prokaryotic cytosol 55
U:Y tRNA (t) Asp/GUC/prokaryotic cytosol 55
U:Y tRNA (t) Cys/GCA/prokaryotic cytosol 55
U:Y tRNA (t) Glu/CUC/prokaryotic cytosol 55
U:Y tRNA (t) Gly/GCC/prokaryotic cytosol 55
U:Y tRNA (t) Gly/{CC/prokaryotic cytosol 55
U:Y tRNA (t) His/GUG/prokaryotic cytosol 55
U:Y tRNA (t) Ile/IAU/prokaryotic cytosol 55
U:Y tRNA (t) Ile/UAU/prokaryotic cytosol 55
U:Y tRNA (t) Leu/UAG/prokaryotic cytosol 55
U:Y tRNA (t) Leu/CAA/prokaryotic cytosol 55
U:Y tRNA (t) Leu/UAA/prokaryotic cytosol 55
U:Y tRNA (t) Lys/CUU/prokaryotic cytosol 55
U:Y tRNA (t) Lys/SUU/prokaryotic cytosol 55
U:Y tRNA (t) Met/CAU/prokaryotic cytosol 55
U:Y tRNA (t) Phe/GAA/prokaryotic cytosol 55
U:Y tRNA (t) Pro/UGG/prokaryotic cytosol 55
U:Y tRNA (t) Ser/CGA/prokaryotic cytosol 55
U:Y tRNA (t) Ser/UGA/prokaryotic cytosol 55
U:Y tRNA (t) Ser/IGA/prokaryotic cytosol 55
U:Y tRNA (t) Thr/IGU/prokaryotic cytosol 55
U:Y tRNA (t) Trp/CCA/prokaryotic cytosol 55
U:Y tRNA (t) Tyr/GUA/prokaryotic cytosol 55
U:Y tRNA (t) Val/UAC/prokaryotic cytosol 55
U:Y tRNA (t) Val/CAC/prokaryotic cytosol 55
U:Y tRNA (t) Val/IAC/prokaryotic cytosol 55
U:Y tRNA (t) Arg/UCU/mitochondrion 55
U:Y tRNA (t) Arg/ACG/mitochondrion 55
U:Y tRNA (t) Gly/UCC/mitochondrion 55
U:Y tRNA (t) His/GUG/mitochondrion 55
U:Y tRNA (t) Ile/GAU/mitochondrion 55
U:Y tRNA (t) Leu/UAA/mitochondrion 55
U:Y tRNA (t) Lys/UUU/mitochondrion 55
U:Y tRNA (t) Met/CAU/mitochondrion 55
U:Y tRNA (t) Phe/GAA/mitochondrion 55
U:Y tRNA (t) Pro/UGG/mitochondrion 55
U:Y tRNA (t) Ser/UGA/mitochondrion 55
U:Y tRNA (t) Ser/GCU/mitochondrion 55
U:Y tRNA (t) Thr/UAG/mitochondrion 55
U:Y tRNA (t) Trp/UCA/mitochondrion 55
U:Y tRNA (t) Tyr/GUA/mitochondrion 55
U:Y tRNA (t)   55

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M N G I F A I E K P S G I T S N Q F M L K L Q H A L T K S Q V F S K E I Q R A T A E R K Q Q Y E K Q T G K K A S K R K L R K V S K V K M G H G G T L D P L A S G V L V I G I G A G T K K L A N Y L S G T V K V Y E S E A L F G V S T T S G D V E G E I L S Q N S V K H L N F D D L K T V E E K F V G Q L K Q T P P I Y A A L K M D G K P L H E Y A R E G K P L P R A I E P R Q V T I Y D L K V F S D S L K R D H D Y P L L R P T T E E A V D T V K N L N A N M L N D V L Y F S K E Y T E K H G L D S E V A K V E E P F P L S E Q E E Q E I Q K E G D S Y R A P K L H F K A N V S S G T Y I R S L V S D I G K S M R S S C Y M V K L I R L Q Q Q D W S L E K N N V F Q L T D F T E R D E K V W S K V L E K V L D E G A T V D V I E E L K K A E K E I P A D V K E C I V S S D Q P G D E A T A E T I E T A N A E E H S N T L K R K I E Q V

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P48567-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P48567-F1.cif  





Publications:

Title Authors Journal Details PubMed Id DOI
The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of psi55 in both mitochondrial and cytoplasmic tRNAs. Becker HF, Motorin Y, Planta RJ, Grosjean H Nucleic Acids Res [details] 9358157 -