Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA 4-thiouridine(8) synthase
Synonym: NuvA
GI: 2501549
Orf: b0423
COG: COG0301
UniProt: P77718
Alpha Fold Predicted Structure: AF-P77718-F1
Enzyme type: sulfurtransferase
Position of modification - modification: t:8 - s4U



Protein sequence:

MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQLEGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLLLIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTEQEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNKTWLLWCERGVMSRLQALYLREQGFNNVKVYRP

Comments:

Requires IcsS as a sulphur donor. Thiamine biosyntheses protein. Contains 1 rhodanese domain. Sulfur is transferred to ThiI through direct binding of IscS. ThiI then transfers the sulfur group to the conserved U8 of bacterial tRNA to form s4U8.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
U:s4U RNA tRNA 8 ICG ICG tRNAArgICG acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 QUU QUU tRNAAsnQUU acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 QUC QUC tRNAAspQUC acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 GCA GCA tRNACysGCA acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 NUG NUG tRNAGlnNUG acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 CUG CUG tRNAGlnCUG acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 CCC CCC tRNAGlyCCC acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 QUG QUG tRNAHisQUG acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 }AU }AU tRNAIle}AU acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 )AA )AA tRNALeu)AA acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 MAU MAU tRNAMetMAU acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 GAA GAA tRNAPheGAA acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 UGA UGA tRNASerUGA acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 GCU GCU tRNASerGCU acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 GGA GGA tRNASerGAA acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 CCA CCA tRNATrpCCA acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 QUA QUA tRNATyrQUA acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 VAC VAC tRNAValVAC acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 GAC GAC tRNAValGAC acceptor-stem Prokaryotic Cytosol
U:s4U RNA tRNA 8 BAA BAA tRNALeuBAA acceptor-stem Prokaryotic Cytosol

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M K F I I K L F P E I T I K S Q S V R L R F I K I L T G N I R N V L K H Y D E T L A V V R H W D N I E V R A K D E N Q R L A I R D A L T R I P G I H H I L E V E D V P F T D M H D I F E K A L V Q Y R D Q L E G K T F C V R V K R R G K H D F S S I D V E R Y V G G G L N Q H I E S A R V K L T N P D V T V H L E V E D D R L L L I K G R Y E G I G G F P I G T Q E D V L S L I S G G F D S G V S S Y M L M R R G C R V H Y C F F N L G G A A H E I G V R Q V A H Y L W N R F G S S H R V R F V A I N F E P V V G E I L E K I D D G Q M G V I L K R M M V R A A S K V A E R Y G V Q A L V T G E A L G Q V S S Q T L T N L R L I D N V S D T L I L R P L I S Y D K E H I I N L A R Q I G T E D F A R T M P E Y C G V I S K S P T V K A V K S K I E A E E E K F D F S I L D K V V E E A N N V D I R E I A Q Q T E Q E V V E V E T V N G F G P N D V I L D I R S I D E Q E D K P L K V E G I D V V S L P F Y K L S T K F G D L D Q N K T W L L W C E R G V M S R L Q A L Y L R E Q G F N N V K V Y R P

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P77718-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P77718-F1.cif  
DSSP Secondary Structures   P77718.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Identification of a gene involved in the generation of 4-thiouridine in tRNA. Mueller EG, Buck CJ, Palenchar PM, Barnhart LE, Paulson JL Nucleic Acids Res [details] 9592144 -
Evidence for the transfer of sulfane sulfur from IscS to ThiI during the in vitro biosynthesis of 4-thiouridine in Escherichia coli tRNA. Kambampati R, Lauhon CT J Biol Chem [details] 10753862 -
Deduced RNA binding mechanism of ThiI based on structural and binding analyses of a minimal RNA ligand. Tanaka Y, Yamagata S, Kitago Y, Yamada Y, Chimnaronk S, Yao M, Tanaka I RNA [details] 19509301 -
Direct evidence that ThiI is an ATP pyrophosphatase for the adenylation of uridine in 4-thiouridine biosynthesis. You D, Xu T, Yao F, Zhou X, Deng Z Chembiochem [details] 18604845 -
Direct evidence for enzyme persulfide and disulfide intermediates during 4-thiouridine biosynthesis. Wright CM, Christman GD, Snellinger AM, Johnston MV, Mueller EG Chem Commun (Camb) [details] 16855700 -

Links:

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