Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA modification GTPase MSS1
Synonym: Pet53
GI: 1709142
Orf: YMR023C
COG: COG0486
UniProt: P32559
Alpha Fold Predicted Structure: AF-P32559-F1
Complex: Mss1/Mto1
Enzyme type: GTPase



Protein sequence:

MNSASFLQSRLISRSFLVRRSLKRYSGLAKPYTFQQPTIYALSTPANQTSAIAIIRISGTHAKYIYNRLVDSSTVPPIRKAILRNIYSPSSCSVKPHDQKESKILLDTSLLLYFQAPYSFTGEDVLELHVHGGKAVVNSILKAIGSLHDRSSGKDIRFALPGDFSRRAFQNGKFDLTQLEGIKDLIDSETESQRRSALSSFNGDNKILFENWRETIIENMAQLTAIIDFADDNSQEIQNTDEIFHNVEKNIICLRDQIVTFMQKVEKSTILQNGIKLVLLGAPNVGKSSLVNSLTNDDISIVSDIPGTTRDSIDAMINVNGYKVIICDTAGIREKSSDKIEMLGIDRAKKKSVQSDLCLFIVDPTDLSKLLPEDILAHLSSKTFGNKRIIIVVNKSDLVSDDEMTKVLNKLQTRLGSKYPILSVSCKTKEGIESLISTLTSNFESLSQSSADASPVIVSKRVSEILKNDVLYGLEEFFKSKDFHNDIVLATENLRYASDGIAKITGQAIGIEEILDSVFSKFCIGK

Comments:

Yeast mitochondrial homolog of bacterial GTPase MnmE. Makes a stable complex with Mto1p. Does not work on cytoplasmic tRNAs.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
s2U:cmnm5s2U RNA tRNA 34 Mitochondrion 17684231    15509579   
U:cmnm5U RNA tRNA 34 Mitochondrion

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M N S A S F L Q S R L I S R S F L V R R S L K R Y S G L A K P Y T F Q Q P T I Y A L S T P A N Q T S A I A I I R I S G T H A K Y I Y N R L V D S S T V P P I R K A I L R N I Y S P S S C S V K P H D Q K E S K I L L D T S L L L Y F Q A P Y S F T G E D V L E L H V H G G K A V V N S I L K A I G S L H D R S S G K D I R F A L P G D F S R R A F Q N G K F D L T Q L E G I K D L I D S E T E S Q R R S A L S S F N G D N K I L F E N W R E T I I E N M A Q L T A I I D F A D D N S Q E I Q N T D E I F H N V E K N I I C L R D Q I V T F M Q K V E K S T I L Q N G I K L V L L G A P N V G K S S L V N S L T N D D I S I V S D I P G T T R D S I D A M I N V N G Y K V I I C D T A G I R E K S S D K I E M L G I D R A K K K S V Q S D L C L F I V D P T D L S K L L P E D I L A H L S S K T F G N K R I I I V V N K S D L V S D D E M T K V L N K L Q T R L G S K Y P I L S V S C K T K E G I E S L I S T L T S N F E S L S Q S S A D A S P V I V S K R V S E I L K N D V L Y G L E E F F K S K D F H N D I V L A T E N L R Y A S D G I A K I T G Q A I G I E E I L D S V F S K F C I G K

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P32559-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P32559-F1.cif  
DSSP Secondary Structures   P32559.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
MSS1, a nuclear-encoded mitochondrial GTPase involved in the expression of COX1 subunit of cytochrome c oxidase. Decoster E, Vassal A, Faye G J Mol Biol [details] 8392589 -
Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases. Umeda N, Suzuki T, Yukawa M, Ohya Y, Shindo H, Watanabe K, Suzuki T J Biol Chem [details] 15509579 -

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