| Full name: | Ribosomal RNA small subunit methyltransferase A |
|---|---|
| Synonym: | KsgA, PA0592 |
| UniProt: | Q9I5U5 |
| Enzyme type: | |
| Position of modification - modification: |
:1518(None) - m6A :1518(None) - m6,6A :1519(None) - m6A :1519(None) - m6,6A |
| Level of experimental evidence: | 3 |
| Level of experimental reliability: | 2 |
MSELYQHRARKRFGQNFLHDAGVIHRILRAIHAREGQRLLEIGPGQGALTEGLLGSGARLDVIELDQDLIPLLKLKFGLESRFSLHQGDALKFDFASLVESGEKLRVVGNLPYNISTPLIFHLLEHAPVIEDMHFMLQKEVVERLAATPGGGDWGRLSIMVQYHCRVEHLFNVGPGAFNPPPKVDSAIVRLTPFAEPPHPARDPKLLERVVREAFNQRRKTLRNTLKPLLSVEDIEAAEVDPTLRPEQLDLAAFVRLANQLAELPGNR
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. Plays the role in KsgA in oxidative stress responses and antibiotic sensitivity in P. aeruginosa.
| Reaction | Substrate | SubstrateType | Position | (Anti)Codon | Modified (Anti)Codon | Amino Acid Change | Transcript Name | Transcript Region | Cellular Localization | References |
|---|---|---|---|---|---|---|---|---|---|---|
| A:m6A | rRNA (r) | rRNA/rRNA/prokaryotic cytosol | 1518 | |||||||
| m6A:m6,6A | rRNA (r) | rRNA/rRNA/prokaryotic cytosol | 1518 | |||||||
| A:m6A | rRNA (r) | rRNA/rRNA/prokaryotic cytosol | 1519 | |||||||
| m6A:m6,6A | rRNA (r) | rRNA/rRNA/prokaryotic cytosol | 1519 |
| Title | Authors | Journal | Details | ||
|---|---|---|---|---|---|
| 16S rRNA methyltransferase KsgA contributes to oxidative stress and antibiotic resistance in Pseudomonas aeruginosa. | Phatinuwat K; Atichartpongkul S; Jumpathong W; Mongkolsuk S; Fuangthong M | Sci Rep | [details] | 39489773 | 10.1038/s41598-024-78296-4 |