Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA (guanine(46)-N7)-methyltransferase
Synonym: YggH
GI: 67473592
Orf: b2960
COG: COG0220
UniProt: P0A8I5
Structures: | 3DXX | 3DXY | 3DXZ |
Alpha Fold Predicted Structure: AF-P0A8I5-F1
Enzyme type: methyltransferase
Position of modification - modification: t:46 - m7G


PDB Structures:


3DXX

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

nan

Download RCSB-PDB Structures:

Pdb Files   3DXX.pdb   3DXY.pdb   3DXZ.pdb  
Pdbx/mmCIF Files   3DXX.cif   3DXY.cif   3DXZ.cif  


Protein sequence:

MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPVMGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGACLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVPFAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTKFEQRGHRLGHGVWDLMFERVK

Comments:

AdoMet is the methyl group donor.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
G:m7G RNA tRNA 46 UGC UGC tRNAAlaUGC V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 GGC GGC tRNAAlaGGC V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 ACG ACG tRNAArgACG V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 CCG CCG tRNAArgCCG V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 GUU GUU tRNAAsnGUU V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 GUC GUC tRNAAspGUC V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 GCC GCC tRNAGlyGCC V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 GUG GUG tRNAHisGUG V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 GAU GAU tRNAIleGAU V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 CAU CAU tRNAIleCAU V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 SUU SUU tRNALysSUU V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 CAU CAU tRNAMetCAU V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 GAA GAA tRNAPheGAA V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 GGU GGU tRNAThrGGU V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 CCA CCA tRNATrpCCA V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 UAC UAC tRNAValUAC V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 GAC GAC tRNAValGAC V-loop Prokaryotic Cytosol
G:m7G RNA tRNA 46 V-loop Prokaryotic Cytosol

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M K N D V I S P E F D E N G R P L R R I R S F V R R Q G R L T K G Q E H A L E N Y W P V M G V E F S E D M L D F P A L F G R E A P V T L E I G F G M G A S L V A M A K D R P E Q D F L G I E V H S P G V G A C L A S A H E E G L S N L R V M C H D A V E V L H K M I P D N S L R M V Q L F F P D P W H K A R H N K R R I V Q V P F A E L V K S K L Q L G G V F H M A T D W E P Y A E H M L E V M S S I D G Y K N L S E S N D Y V P R P A S R P V T K F E Q R G H R L G H G V W D L M F E R V K

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P0A8I5-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P0A8I5-F1.cif  
DSSP Secondary Structures   P0A8I5.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase. De Bie LG, Roovers M, Oudjama Y, Wattiez R, Tricot C, Stalon V, Droogmans L, Bujnicki JM J Bacteriol [details] 12730187 -
Sequence-structure-function relationships of a tRNA (m7G46) methyltransferase studied by homology modeling and site-directed mutagenesis. Purta E, van Vliet F, Tricot C, De Bie LG, Feder M, Skowronek K, Droogmans L, Bujnicki JM Proteins [details] 15789416 -