Modomics - A Database of RNA Modifications

ID Card:

Full name: RNA cap guanine-N2 methyltransferase
GI: 159108485
Orf: GL50803_5613
COG: COG2265
UniProt: A8BW33
Alpha Fold Predicted Structure: AF-A8BW33-F1
Enzyme type: methyltransferase
Position of modification - modification: n:0 - m2,2,7GpppN
snoRNA:0 - m2,2,7GpppN



Protein sequence:

MSTWLLDSKCVERMKWLFSDLPEEKRVMIKMNEVAFFSVTPAVYADEVARMMRTVLALLGKPPYAVIDGTACVGGDTRLLAKHFDMTVAIERDPETYALLQDNLTTWGVDAKTISGDTAALIPQFWTLIGAVATFSLYLDPPWGGVDYRSQTDIQLTLGSLAVEDVVNRAFEAHLSMKLAVLKLPRNYNCGYLFRKLGKHEVFRITQGNFFVFFVARRGSRVKEHGRTAMLQLRKAREEAKARSEETKEDGETRGSGE

Comments:

Tgs1 is the enzyme responsible for converting 7-methyl-guanosine RNA caps to the 2,2,7-trimethylguanosine cap structures of small nuclear and small nucleolar RNAs. In comparison to budding yeast S.cerevisiae and fission yeast S.pombe encode a single Tgs1 protein, the primitive eukaryote Giardia lamblia encodes two paralogs, Tgs1 and Tgs2. Tgs2 is a monomeric enzyme that catalyzes methyl transfer from AdoMet to m7GDP to form m2,7GDP. Tgs2 also methylates m7GTP and m7GpppA but is unreactive with GDP, GTP, GpppA, ATP, CTP, or UTP. Tgs2's sp-68, Glu-91, and Trp-143 are essential for Tgs2 methyltransferase activity in vitro (Hausmann et al. 2007 ).




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
m7G:m2,7G RNA snoRNA 1 5' - end Nucleolus 17284461   
m7G:m2,7G RNA snRNA 1 5' - end Nucleus 17284461   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M S T W L L D S K C V E R M K W L F S D L P E E K R V M I K M N E V A F F S V T P A V Y A D E V A R M M R T V L A L L G K P P Y A V I D G T A C V G G D T R L L A K H F D M T V A I E R D P E T Y A L L Q D N L T T W G V D A K T I S G D T A A L I P Q F W T L I G A V A T F S L Y L D P P W G G V D Y R S Q T D I Q L T L G S L A V E D V V N R A F E A H L S M K L A V L K L P R N Y N C G Y L F R K L G K H E V F R I T Q G N F F V F F V A R R G S R V K E H G R T A M L Q L R K A R E E A K A R S E E T K E D G E T R G S G E

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-A8BW33-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-A8BW33-F1.cif  





Publications:

Title Authors Journal Details PubMed Id DOI
Giardia lamblia RNA cap guanine-N2 methyltransferase (Tgs2). Hausmann S, Shuman S J Biol Chem [details] 16046409 -
Biochemical and genetic analysis of RNA cap guanine-N2 methyltransferases from Giardia lamblia and Schizosaccharomyces pombe. Hausmann S, Ramirez A, Schneider S, Schwer B, Shuman S Nucleic Acids Res [details] 17284461 -