Modomics - A Database of RNA Modifications

ID Card:

Full name: 28S rRNA (cytosine-C(5))-methyltransferase
UniProt: Q8K4F6
Alpha Fold Predicted Structure: AF-Q8K4F6-F1
Enzyme type: methyltransferase



Protein sequence:

MGLYAAAAAVLAGVESRQGSLKGLVYSSNFQNLKQLYALVCETQRYSAVLDAVIASAGLLRAEKKLRPHLAKVLVYELLLGKGFRGGGGRWKALLGRHQARLKAELARLKVHRGVSRNEDLLQESSRPGQAYQVPRFVRVNTLKTRPEDAIDYFKRQGFSYQGRASSLEDLRALKGQHFLLDPLLPELLVFPAQTDLHEHPLYRAGHLILQDKASCLPAMLLSPPPGSHVIDACAAPGNKTSYIAALLKNQGKIFAFDQDAKRLAAMATLVARAGVSCCELAEKDFLTVSPSDQRYSQVQYILLDPSCSGSGMLSRQLEEHGEGTPSKERLQALAGFQQRALCHALRFPSLQRLVYSTCSLCQEENEDVVQEALQHNSGTFRLAPVLPTWPHRGLSTFPGSEHCLRASPETTLTGGFFIAVFERAEVVPTPAPQTDAMDPEPLSQVPKRKRRRKAAVGASMQPST

Comments:

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 3438 (m5C3438) in 28S rRNA (Heissenberger et al. 2019 ). m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity.





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M G L Y A A A A A V L A G V E S R Q G S L K G L V Y S S N F Q N L K Q L Y A L V C E T Q R Y S A V L D A V I A S A G L L R A E K K L R P H L A K V L V Y E L L L G K G F R G G G G R W K A L L G R H Q A R L K A E L A R L K V H R G V S R N E D L L Q E S S R P G Q A Y Q V P R F V R V N T L K T R P E D A I D Y F K R Q G F S Y Q G R A S S L E D L R A L K G Q H F L L D P L L P E L L V F P A Q T D L H E H P L Y R A G H L I L Q D K A S C L P A M L L S P P P G S H V I D A C A A P G N K T S Y I A A L L K N Q G K I F A F D Q D A K R L A A M A T L V A R A G V S C C E L A E K D F L T V S P S D Q R Y S Q V Q Y I L L D P S C S G S G M L S R Q L E E H G E G T P S K E R L Q A L A G F Q Q R A L C H A L R F P S L Q R L V Y S T C S L C Q E E N E D V V Q E A L Q H N S G T F R L A P V L P T W P H R G L S T F P G S E H C L R A S P E T T L T G G F F I A V F E R A E V V P T P A P Q T D A M D P E P L S Q V P K R K R R R K A A V G A S M Q P S T

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q8K4F6-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q8K4F6-F1.cif  
DSSP Secondary Structures   Q8K4F6.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Loss of the ribosomal RNA methyltransferase NSUN5 impairs global protein synthesis and normal growth Clemens Heissenberger 1, Lisa Liendl 1, Fabian Nagelreiter 1, Yulia Gonskikh 2, Guohuan Yang 3, Elena M Stelzer 1, Teresa L Krammer 1, Lucia Micutkova 4, Stefan Vogt 1, David P Kreil 1, Gerhard Sekot 1, Emilio Siena 1, Ina Poser 5, Eva Harreither 1, Angela Linder 1, Viktoria Ehret 6, Thomas H Helbich 6, Regina Grillari-Voglauer 1, Pidder Jansen-Dürr 4, Martin Koš 3, Norbert Polacek 2, Johannes Grillari 1 7 8, Markus Schosserer [details] 31722427 10.1093/nar/gkz1043

Links:

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