Modomics - A Database of RNA Modifications

ID Card:

Full name: RNA phosphate cyclase
GI: 12644279
UniProt: P46849
Structures: | 1QHM | 1QMI | 3KGD | 3TUT | 3TUX | 3TV1 | 3TW3 |
Alpha Fold Predicted Structure: AF-P46849-F1
Enzyme type: cyclase


PDB Structures:


1QHM

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

Pyruvate formate lyase (PFL) catalyses a key step in Escherichia coli anaerobic glycolysis by converting pyruvate and CoA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate, involving an essential C alpha radical of Gly734 and two cysteine residues, Cys418 and Cys419, which may form thiyl radicals required for catalysis. We undertook this study to understand the structural basis for catalysis.

Download RCSB-PDB Structures:

Pdb Files   1QHM.pdb   1QMI.pdb   3KGD.pdb   3TUT.pdb   3TUX.pdb   3TV1.pdb   3TW3.pdb  
Pdbx/mmCIF Files   1QHM.cif   1QMI.cif   3KGD.cif   3TUT.cif   3TUX.cif   3TV1.cif   3TW3.cif  


Protein sequence:

MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAKPGLLRQHLTAVKAATEICGATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVLQTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFYPAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSLHEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASTAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTRVSIE

Comments:

RtcA catalyses adenylylation of 5'-p ends of DNA or RNA strands using RtcA-histidinyl-N-AMP intermediate to form A-pp-DNA and A-pp-RNA product. RtcA is coregulated in an operon with an RNA ligase, RtcB, that splices RNA 5'-OH ends to either 3'-phosphate or 2',3'-cyclic phosphate ends. RtcA might serve an end healing function in an RNA repair pathway, by converting RNA 2'-phosphates, which cannot be spliced by RtcB, to 2',3'-cyclic phosphates that can be sealed.





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M K R M I A L D G A Q G E G G G Q I L R S A L S L S M I T G Q P F T I T S I R A G R A K P G L L R Q H L T A V K A A T E I C G A T V E G A E L G S Q R L L F R P G T V R G G D Y R F A I G S A G S C T L V L Q T V L P A L W F A D G P S R V E V S G G T D N P S A P P A D F I R R V L E P L L A K I G I H Q Q T T L L R H G F Y P A G G G V V A T E V S P V A S F N T L Q L G E R G N I V Q M R G E V L L A G V P R H V A E R E I A T L A G S F S L H E Q N I H N L P R D Q G P G N T V S L E V E S E N I T E R F F V V G E K R V S A E V V A A Q L V K E V K R Y L A S T A A V G E Y L A D Q L V L P M A L A G A G E F T V A H P S C H L L T N I A V V E R F L P V R F S L I E T D G V T R V S I E

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P46849-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P46849-F1.cif  
DSSP Secondary Structures   P46849.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Local cerebral glucose consumption during ethanol withdrawal in the rat: effects of single and multiple episodes and previous convulsive seizures. L Clemmesen,M Ingvar,R Hemmingsen,T G Bolwig Brain Res [details] 3401759 -