Modomics - A Database of RNA Modifications

ID Card:

Full name:	N6-adenosine-methyltransferase MT-A70-like
GI:	1063721623
UniProt:	O82486
Alpha Fold Predicted Structure:	AF-O82486-F1
Enzyme type:	methyltransferase

Protein sequence:

METESDDATITVVKDMRVRLENRIRTQHDAHLDLLSSLQSIVPDIVPSLDLSLKLISSFTNRPFVATPPLPEPKVEKKHHPIVKLGTQLQQLHGHDSKSMLVDSNQRDAEADGSSGSPMALVRAMVAECLLQRVPFSPTDSSTVLRKLENDQNARPAEKAALRDLGGECGPILAVETALKSMAEENGSVELEEFEVSGKPRIMVLAIDRTRLLKELPESFQGNNESNRVVETPNSIENATVSGGGFGVSGSGNFPRPEMWGGDPNMGFRPMMNAPRGMQMMGMHHPMGIMGRPPPFPLPLPLPVPSNQKLRSEEEDLKDVEALLSKKSFKEKQQSRTGEELLDLIHRPTAKEAATAAKFKSKGGSQVKYYCRYLTKEDCRLQSGSHIACNKRHFRRLIASHTDVSLGDCSFLDTCRHMKTCKYVHYELDMADAMMAGPDKALKPLRADYCSEAELGEAQWINCDIRSFRMDILGTFGVVMADPPWDIHMELPYGTMADDEMRTLNVPSLQTDGLIFLWVTGRAMELGRECLELWGYKRVEEIIWVKTNQLQRIIRTGRTGHWLNHSKEHCLVGIKGNPEVNRNIDTDVIVAEVRETSRKPDEMYAMLERIMPRARKLELFARMHNAHAGWLSLGNQLNGVRLINEGLRARFKASYPEIDVQPPSPPRASAMETDNEPMAIDSITA

Comments:

The components required for m6A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m6A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m6A methylation plays a role in developmental decisions during pattern formation.

Alpha Fold Predicted Structure:

Clear Selection and Reset Camera

Protein sequence:

M E T E S D D A T I T V V K D M R V R L E N R I R T Q H D A H L D L L S S L Q S I V P D I V P S L D L S L K L I S S F T N R P F V A T P P L P E P K V E K K H H P I V K L G T Q L Q Q L H G H D S K S M L V D S N Q R D A E A D G S S G S P M A L V R A M V A E C L L Q R V P F S P T D S S T V L R K L E N D Q N A R P A E K A A L R D L G G E C G P I L A V E T A L K S M A E E N G S V E L E E F E V S G K P R I M V L A I D R T R L L K E L P E S F Q G N N E S N R V V E T P N S I E N A T V S G G G F G V S G S G N F P R P E M W G G D P N M G F R P M M N A P R G M Q M M G M H H P M G I M G R P P P F P L P L P L P V P S N Q K L R S E E E D L K D V E A L L S K K S F K E K Q Q S R T G E E L L D L I H R P T A K E A A T A A K F K S K G G S Q V K Y Y C R Y L T K E D C R L Q S G S H I A C N K R H F R R L I A S H T D V S L G D C S F L D T C R H M K T C K Y V H Y E L D M A D A M M A G P D K A L K P L R A D Y C S E A E L G E A Q W I N C D I R S F R M D I L G T F G V V M A D P P W D I H M E L P Y G T M A D D E M R T L N V P S L Q T D G L I F L W V T G R A M E L G R E C L E L W G Y K R V E E I I W V K T N Q L Q R I I R T G R T G H W L N H S K E H C L V G I K G N P E V N R N I D T D V I V A E V R E T S R K P D E M Y A M L E R I M P R A R K L E L F A R M H N A H A G W L S L G N Q L N G V R L I N E G L R A R F K A S Y P E I D V Q P P S P P R A S A M E T D N E P M A I D S I T A

Secondary Structure Alphabet

G: 3-turn helix (3₁₀helix)
H: α-helix
I: 𝝅-helix (5 - turn helix)
T: Hydrogen Bonded Turn
B: β-sheet
S: Bend
C: Coil (residues not present in any of the above conformations)
N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files	AF-O82486-F1.pdb
Alpha Fold Pdbx/mmCIF Files	AF-O82486-F1.cif
DSSP Secondary Structures	O82486.dssp

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
MTA is an Arabidopsis messenger RNA adenosine methylase and interacts with a homolog of a sex-specific splicing factor.	Zhong S, Li H, Bodi Z, Button J, Vespa L, Herzog M, Fray RG	Plant Cell	[details]	18505803	10.1105/tpc.108.058883
Identification of factors required for m(6) A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI.	Růžička K, Zhang M, Campilho A, Bodi Z, Kashif M, Saleh M, Eeckhout D, El-Showk S, Li H, Zhong S, De Jaeger G, Mongan NP, Hejátko J, Helariutta Y, Fray RG	New Phytol.	[details]	28503769	10.1111/nph.14586
Unique Features of the m6A Methylome in Arabidopsis thaliana	Guan-Zheng Luo, Alice MacQueen, Guanqun Zheng, Hongchao Duan, Louis C Dore, Zhike Lu, Jun Liu, Kai Chen, Guifang Jia, Joy Bergelson, Chuan He	Nat Commun.	[details]	25430002	10.1038/ncomms6630