Modomics - A Database of RNA Modifications

ID Card:

Full name: glutamine:preQ0-tRNA amidinotransferase or Archaeosine Synthase
Synonym: Archaeosine synthase, Glutamine:preQ0-tRNA amidinotransferase
GI: 3024970
COG: COG1549
UniProt: Q58428
Alpha Fold Predicted Structure: AF-Q58428-F1
Enzyme type: amidinotransferase
Position of modification - modification: t:15 - G+



Protein sequence:

MLEPIAYDIGRLCKEEDKELTPKLIDIDVIGLSQEKIFYGIMTPFRCPNSKSIYELRKSYVKADGIKMPFDTFRELTSIFKKSFIGTVKYKGNVFKYQILNFGKHVDLIELEDADLYIIADGRRLIERKELQIIPKIREKISPNSAIYSPAVFPWEIPLLAYIGVDYFDDSLAKLYASMGYKFTKNRAVKVDSFSFEELYNNNKKVYEEILEEVRIAIKNGFLRNVVEETAVSHPYLWANYRRYEPDLRNIPLSKENKIIVTTNINIPEVKKYLERLDNYEPYSNIIVLLPCSSKKPYSISQSHQKFIKAIKSAKVVVEEVILTSPYGLVPRALERLVNYDIPVTGEWSFEEIELINNCLKNFLKKVKEKFDDYIVIAHLPEHYLEILELDDIVITSKGNPTSEEALKNLTDTLKKYKELTKSKDINKKGQRIHNIQQLAEFQFGINFIPNEIFINHKGQIFTKINNKNQQIASINPKNGLLILTLSGGELLWNSGGKDINYIEVNYEIKKGSLFPPGFVDCNENISYNDEVVLIKDDTFLGIGRALMSGFEMKKAKHGALVNIRNVKS

Comments:

7-deazaguanosine derivative archaeosine (G(+)) at tRNA position 15 is a molecular signature of Archaea. Noteworthy, the biosynthesis of this modified ribonucleotide is complex, involving the initial production of 7-cyano-7-deazaguanine (preQ(0)). ArcTGTs are an enzyme family responsible for the biosynthesis of pre Q(0). The corresponding gene has been cloned also for Methano caldococcus jannaschii (Philips et al. 2010 ). It was shown to convert pre Q(0) to G(+)-tRNA using several nitrogen sources and to do so in an ATP dependent process.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
preQ0:G+ RNA tRNA 15 preQ0 - tRNA D-loop cytosol 20129918   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M L E P I A Y D I G R L C K E E D K E L T P K L I D I D V I G L S Q E K I F Y G I M T P F R C P N S K S I Y E L R K S Y V K A D G I K M P F D T F R E L T S I F K K S F I G T V K Y K G N V F K Y Q I L N F G K H V D L I E L E D A D L Y I I A D G R R L I E R K E L Q I I P K I R E K I S P N S A I Y S P A V F P W E I P L L A Y I G V D Y F D D S L A K L Y A S M G Y K F T K N R A V K V D S F S F E E L Y N N N K K V Y E E I L E E V R I A I K N G F L R N V V E E T A V S H P Y L W A N Y R R Y E P D L R N I P L S K E N K I I V T T N I N I P E V K K Y L E R L D N Y E P Y S N I I V L L P C S S K K P Y S I S Q S H Q K F I K A I K S A K V V V E E V I L T S P Y G L V P R A L E R L V N Y D I P V T G E W S F E E I E L I N N C L K N F L K K V K E K F D D Y I V I A H L P E H Y L E I L E L D D I V I T S K G N P T S E E A L K N L T D T L K K Y K E L T K S K D I N K K G Q R I H N I Q Q L A E F Q F G I N F I P N E I F I N H K G Q I F T K I N N K N Q Q I A S I N P K N G L L I L T L S G G E L L W N S G G K D I N Y I E V N Y E I K K G S L F P P G F V D C N E N I S Y N D E V V L I K D D T F L G I G R A L M S G F E M K K A K H G A L V N I R N V K S

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q58428-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q58428-F1.cif  
DSSP Secondary Structures   Q58428.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Discovery and characterization of an amidinotransferase involved in the modification of archaeal tRNA. Phillips G, Chikwana VM, Maxwell A, El-Yacoubi B, Swairjo MA, Iwata-Reuyl D, de Crecy-Lagard V... J Biol Chem [details] 20129918 -