ID Card:
Full name:
25S rRNA (uridine(2843)-N(3))-methyltransferase
Synonym:
YLR063W
GI:
6323092
Orf:
L2174
UniProt:
Q12291
Alpha Fold Predicted Structure:
AF-Q12291-F1
Enzyme type:
methyltransferase
Position of modification - modification:
l :2843(2474) - m3U
Protein sequence:
MLLMRRFAFLTSSVYFKYIPIYSQYHYSSQFPINMNPKKVAQLPVHNKSTLPPQEIIDLFKITFLEELYPKDQDNEKSPLTEQIQAVKSDLYNRDYNAAFNNDSKRIAYCCRWSPSRATSYASVFAHFPELLKIIRCEIDDKDSNVLCIGGGAGGELVALASIFTLSRDFSSKFASALKIDNEVNKKPRNLNIQLVDIADWSTVVEKLTATIKSKWLYGDSEAESFNVNFTHKDCLQMTEPQDIKIYQGLDLITLLFTTNELFTQKKVESIKFLQRLNENCAPGCHLLILESAGSYSHITINNKKFPIQFLIDTILVGNRKDKGTTGPWSLVSENDSIWYRMDPKLDYSIPLENMRFFYRLYVKN
Comments:
Btm6 belongs to the Rossmann-fold-like methyltransferases. Point mutations in the S-adenosyl-L-methionine binding pocket abolish the methylation reaction. Bmt5 localizes predominantly in the cytoplasm. Bmt6 methylates the N3 position of uridine 2843 (m3U2843) in the 25s rRNA (Sharma et al. 2014 )
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
U:m3U
RNA
rRNA
2843
LSU-25S
cytoplasm
24335083
Alpha Fold Predicted Structure:
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Protein sequence:
M
L
L
M
R
R
F
A
F
L
T
S
S
V
Y
F
K
Y
I
P
I
Y
S
Q
Y
H
Y
S
S
Q
F
P
I
N
M
N
P
K
K
V
A
Q
L
P
V
H
N
K
S
T
L
P
P
Q
E
I
I
D
L
F
K
I
T
F
L
E
E
L
Y
P
K
D
Q
D
N
E
K
S
P
L
T
E
Q
I
Q
A
V
K
S
D
L
Y
N
R
D
Y
N
A
A
F
N
N
D
S
K
R
I
A
Y
C
C
R
W
S
P
S
R
A
T
S
Y
A
S
V
F
A
H
F
P
E
L
L
K
I
I
R
C
E
I
D
D
K
D
S
N
V
L
C
I
G
G
G
A
G
G
E
L
V
A
L
A
S
I
F
T
L
S
R
D
F
S
S
K
F
A
S
A
L
K
I
D
N
E
V
N
K
K
P
R
N
L
N
I
Q
L
V
D
I
A
D
W
S
T
V
V
E
K
L
T
A
T
I
K
S
K
W
L
Y
G
D
S
E
A
E
S
F
N
V
N
F
T
H
K
D
C
L
Q
M
T
E
P
Q
D
I
K
I
Y
Q
G
L
D
L
I
T
L
L
F
T
T
N
E
L
F
T
Q
K
K
V
E
S
I
K
F
L
Q
R
L
N
E
N
C
A
P
G
C
H
L
L
I
L
E
S
A
G
S
Y
S
H
I
T
I
N
N
K
K
F
P
I
Q
F
L
I
D
T
I
L
V
G
N
R
K
D
K
G
T
T
G
P
W
S
L
V
S
E
N
D
S
I
W
Y
R
M
D
P
K
L
D
Y
S
I
P
L
E
N
M
R
F
F
Y
R
L
Y
V
K
N
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae.
Sharma S, Yang J, Duttmann S, Watzinger P, Kotter P, Entian KD...
Nucleic Acids Res
[details]
24335083
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