Modomics - A Database of RNA Modifications

ID Card:

Full name: S-adenosyl-L-methionine-dependent methyltransferase
Synonym: YBR141C
GI: 330443457
Orf: YBR1118
UniProt: P38278
Alpha Fold Predicted Structure: AF-P38278-F1
Enzyme type: methyltransferase
Position of modification - modification: l:2142(1784) - m1A



Protein sequence:

MHSRKSKSITGKRKQVGSNVTRVIKPQKTRRIIRRFHHLINKRQSICKFLCLKENLDDSNEEKNDKIIRLSIKGNVRLGKYYEDGKSQSFNDAMESQLLRLHSLIKNESKSKDTSDLAVMYTLLGYIMNQINKLGGLETYQIASQNGQLKERGGDTSKLLEKWIRSSFENCPGAVALEIGSLSSGNRISRCALFRNVVRIDLEEHEGVIKQDFMERPLPRNENDKFDLISCSLVLNFVKNHRDRGAMCHRMVKFLKPQGYIFIVLPQACVTHSRYCDKTLLQNLLGSIGLIMLNSHQSNKLYYCLYQLQVVPPQPSSFSKRIKVNDGPGLNNFGITL

Comments:

S-adenosyl-L-methionine-dependant methyltransferase specifically methylates the N1 position of A2142 in the rRNA LSU-25s. It is predicted to be an Ado-Met dependent methyltransferase from the Rossmann fold superfamily. Noteworthy, LSU-25s is in the domain IV helix 65 region of LSU-25s that accounts for most of the surface of LSU-25s. The loss of m1A2142 modification confers anisomycin and peroxide sensitivity to the cells (Sharma et al. 2013 ).





Alpha Fold Predicted Structure:




Parsing response... [315041/315041]


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Protein sequence:

M H S R K S K S I T G K R K Q V G S N V T R V I K P Q K T R R I I R R F H H L I N K R Q S I C K F L C L K E N L D D S N E E K N D K I I R L S I K G N V R L G K Y Y E D G K S Q S F N D A M E S Q L L R L H S L I K N E S K S K D T S D L A V M Y T L L G Y I M N Q I N K L G G L E T Y Q I A S Q N G Q L K E R G G D T S K L L E K W I R S S F E N C P G A V A L E I G S L S S G N R I S R C A L F R N V V R I D L E E H E G V I K Q D F M E R P L P R N E N D K F D L I S C S L V L N F V K N H R D R G A M C H R M V K F L K P Q G Y I F I V L P Q A C V T H S R Y C D K T L L Q N L L G S I G L I M L N S H Q S N K L Y Y C L Y Q L Q V V P P Q P S S F S K R I K V N D G P G L N N F G I T L
50100150200250300SequenceGHTBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P38278-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P38278-F1.cif  
DSSP Secondary Structures   P38278.dssp  





Publications: