Modomics - A Database of RNA Modifications

ID Card:

Full name: Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2
Synonym: hMTr2, FTSJD1, FLJ11171
GI: 269849596
Orf: FTSJD1, FLJ11171
COG: COG0293
UniProt: Q8IYT2
Alpha Fold Predicted Structure: AF-Q8IYT2-F1
Enzyme type: methyltransferase
Position of modification - modification: m:2 - GpppNNm m:2 - GpppNmNm m:2 - m7GpppNNm m:2 - m7GpppNmNm n:2 - GpppNNm n:2 - GpppNmNm n:2 - m7GpppNNm n:2 - m7GpppNmNm



Protein sequence:

MSKCRKTPVQQLASPASFSPDILADIFELFAKNFSYGKPLNNEWQLPDPSEIFTCDHTELNAFLDLKNSLNEVKNLLSDKKLDEWHEHTAFTNKAGKIISHVRKSVNAELCTQAWCKFHEILCSFPLIPQEAFQNGKLNSLHLCEAPGAFIASLNHYLKSHRFPCHWSWVANTLNPYHEANDDLMMIMDDRLIANTLHWWYFGPDNTGDIMTLKFLTGLQNFISSMATVHLVTADGSFDCQGNPGEQEALVSSLHYCEVVTALTTLGNGGSFVLKMFTMFEHCSINLMYLLNCCFDQVHVFKPATSKAGNSEVYVVCLHYKGREAIHPLLSKMTLNFGTEMKRKALFPHHVIPDSFLKRHEECCVFFHKYQLETISENIRLFECMGKAEQEKLNNLRDCAIQYFMQKFQLKHLSRNNWLVKKSSIGCSTNTKWFGQRNKYFKTYNERKMLEALSWKDKVAKGYFNSWAEEHGVYHPGQSSILEGTASNLECHLWHILEGKKLPKVKCSPFCNGEILKTLNEAIEKSLGGAFNLDSKFRPKQQYSCSCHVFSEELIFSELCSLTECLQDEQVVVPSNQIKCLLVGFSTLRNIKMHIPLEVRLLESAELTTFSCSLLHDGDPTYQRLFLDCLLHSLRELHTGDVMILPVLSCFTRFMAGLIFVLHSCFRFITFVCPTSSDPLRTCAVLLCVGYQDLPNPVFRYLQSVNELLSTLLNSDSPQQVLQFVPMEVLLKGALLDFLWDLNAAIAKRHLHFIIQREREEIINSLQLQN

Comments:

hMTr2 protein transfers a methyl group from S-adenosylmethionine to the 2'-O-ribose of the second nucleotide of messenger RNA and small nuclear RNA. Neither N(7) methylation of the guanosine cap nor 2'-O-ribose methylation of the first transcribed nucleotide are required for hMTr2, but the presence of cap1 methylation increases hMTr2 activity. The hMTr2 protein is distributed throughout the nucleus and cytosol, in contrast to the nuclear hMTr1.




ID Reaction Substrate SubstrateType Transcript Name Transcript Region Cellular Localization References
GpppNN:GpppNNm RNA snRNA 2 small nuclear RNA component of U2 snRNP capG : cap2 Cytoplasm and Nucleoplasm 21310715   
GpppNN:GpppNNm RNA mRNA 2 capG : cap2 Cytoplasm and Nucleoplasm 21310715   
m7GpppNN:m7GpppNNm RNA snRNA 2 small nuclear RNA component of U2 snRNP capG : cap2 Cytoplasm and Nucleoplasm 21310715   
m7GpppNN:m7GpppNNm RNA mRNA 2 capG : cap2 Cytoplasm and Nucleoplasm 21310715   
GpppNmN:GpppNmNm RNA snRNA 2 small nuclear RNA component of U2 snRNP capG : cap2 Cytoplasm and Nucleoplasm 21310715   
GpppNmN:GpppNmNm RNA mRNA 2 capG : cap2 Cytoplasm and Nucleoplasm 21310715   
m7GpppNmN:GpppNmNm RNA snRNA 2 small nuclear RNA component of U2 snRNP capG : cap2 Cytoplasm and Nucleoplasm 21310715   
m7GpppNmN:m7GpppNmNm RNA mRNA 2 capG : cap2 Cytoplasm and Nucleoplasm 21310715   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M S K C R K T P V Q Q L A S P A S F S P D I L A D I F E L F A K N F S Y G K P L N N E W Q L P D P S E I F T C D H T E L N A F L D L K N S L N E V K N L L S D K K L D E W H E H T A F T N K A G K I I S H V R K S V N A E L C T Q A W C K F H E I L C S F P L I P Q E A F Q N G K L N S L H L C E A P G A F I A S L N H Y L K S H R F P C H W S W V A N T L N P Y H E A N D D L M M I M D D R L I A N T L H W W Y F G P D N T G D I M T L K F L T G L Q N F I S S M A T V H L V T A D G S F D C Q G N P G E Q E A L V S S L H Y C E V V T A L T T L G N G G S F V L K M F T M F E H C S I N L M Y L L N C C F D Q V H V F K P A T S K A G N S E V Y V V C L H Y K G R E A I H P L L S K M T L N F G T E M K R K A L F P H H V I P D S F L K R H E E C C V F F H K Y Q L E T I S E N I R L F E C M G K A E Q E K L N N L R D C A I Q Y F M Q K F Q L K H L S R N N W L V K K S S I G C S T N T K W F G Q R N K Y F K T Y N E R K M L E A L S W K D K V A K G Y F N S W A E E H G V Y H P G Q S S I L E G T A S N L E C H L W H I L E G K K L P K V K C S P F C N G E I L K T L N E A I E K S L G G A F N L D S K F R P K Q Q Y S C S C H V F S E E L I F S E L C S L T E C L Q D E Q V V V P S N Q I K C L L V G F S T L R N I K M H I P L E V R L L E S A E L T T F S C S L L H D G D P T Y Q R L F L D C L L H S L R E L H T G D V M I L P V L S C F T R F M A G L I F V L H S C F R F I T F V C P T S S D P L R T C A V L L C V G Y Q D L P N P V F R Y L Q S V N E L L S T L L N S D S P Q Q V L Q F V P M E V L L K G A L L D F L W D L N A A I A K R H L H F I I Q R E R E E I I N S L Q L Q N

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q8IYT2-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q8IYT2-F1.cif  
DSSP Secondary Structures   Q8IYT2.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
2'-O-ribose methylation of cap2 in human: function and evolution in a horizontally mobile family. Werner M, Purta E, Kaminska KH, Cymerman IA, Campbell DA, Mittra B, Zamudio JR, Sturm NR, Jaworski J, Bujnicki JM Nucleic Acids Res [details] 21310715 -

Links:

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