Modomics - A Database of RNA Modifications

ID Card:

Full name: RNA cap guanine-N7 methyltransferase
Synonym: mRNA cap methyltransferase, RG7MT1, hCMT1, hMet
GI: 74735378
Orf: RNMT, KIAA0398
COG: COG2226
UniProt: O43148
Structures: | 3EPP | 3BGV |
Alpha Fold Predicted Structure: AF-O43148-F1
Enzyme type: methyltransferase
Position of modification - modification: m:1 - m7GpppN


PDB Structures:


3EPP

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:



Download RCSB-PDB Structures:

Pdb Files   3BGV.pdb   3EPP.pdb  
Pdbx/mmCIF Files   3BGV.cif   3EPP.cif  


Protein sequence:

MANSAKAEEYEKMSLEQAKASVNSETESSFNINENTTASGTGLSEKTSVCRQVDIARKRKEFEDDLVKESSSCGKDTPSKKRKLDPEIVPEEKDCGDAEGNSKKRKRETEDVPKDKSSTGDGTQNKRKIALEDVPEKQKNLEEGHSSTVAAHYNELQEVGLEKRSQSRIFYLRNFNNWMKSVLIGEFLEKVRQKKKRDITVLDLGCGKGGDLLKWKKGRINKLVCTDIADVSVKQCQQRYEDMKNRRDSEYIFSAEFITADSSKELLIDKFRDPQMCFDICSCQFVCHYSFESYEQADMMLRNACERLSPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKGDYPLFGCKYDFNLEGVVDVPEFLVYFPLLNEMAKKYNMKLVYKKTFLEFYEEKIKNNENKMLLKRMQALEPYPANESSKLVSEKVDDYEHAAKYMKNSQVRLPLGTLSKSEWEATSIYLVFAFEKQQ

Comments:

RNMT catalyzes methylation of the cap at the N-7 position to create the methyl cap, m7G(5')ppp(5')X. RNMT can only catalyze methylation of guanosine when it is a component of a cap structure attached to a transcript. RAM (RNMT-Activating Mini protein)/Fam103a1 is required for efficient cap methylation in vitro and in vivo, and is indirectly required to maintain mRNA expression levels, for mRNA translation and for cell viability.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
GpppN:m7GpppN mRNA (m) 1 9705270   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M A N S A K A E E Y E K M S L E Q A K A S V N S E T E S S F N I N E N T T A S G T G L S E K T S V C R Q V D I A R K R K E F E D D L V K E S S S C G K D T P S K K R K L D P E I V P E E K D C G D A E G N S K K R K R E T E D V P K D K S S T G D G T Q N K R K I A L E D V P E K Q K N L E E G H S S T V A A H Y N E L Q E V G L E K R S Q S R I F Y L R N F N N W M K S V L I G E F L E K V R Q K K K R D I T V L D L G C G K G G D L L K W K K G R I N K L V C T D I A D V S V K Q C Q Q R Y E D M K N R R D S E Y I F S A E F I T A D S S K E L L I D K F R D P Q M C F D I C S C Q F V C H Y S F E S Y E Q A D M M L R N A C E R L S P G G Y F I G T T P N S F E L I R R L E A S E T E S F G N E I Y T V K F Q K K G D Y P L F G C K Y D F N L E G V V D V P E F L V Y F P L L N E M A K K Y N M K L V Y K K T F L E F Y E E K I K N N E N K M L L K R M Q A L E P Y P A N E S S K L V S E K V D D Y E H A A K Y M K N S Q V R L P L G T L S K S E W E A T S I Y L V F A F E K Q Q

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-O43148-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-O43148-F1.cif  
DSSP Secondary Structures   O43148.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes. Pillutla RC, Yue Z, Maldonado E, Shatkin AJ J Biol Chem [details] 9705270 -
Cloning and characterization of three human cDNAs encoding mRNA (guanine-7-)-methyltransferase, an mRNA cap methylase. Tsukamoto T, Shibagaki Y, Niikura Y, Mizumoto K Biochem Biophys Res Commun [details] 9790902 -
Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes. Saha N, Schwer B, Shuman S J Biol Chem [details] 10347220 -
Cap methyltransferase selective binding and methylation of GpppG-RNA are stimulated by importin-alpha. Wen Y, Shatkin AJ Genes Dev [details] 11114884 -
Human mRNA cap methyltransferase: alternative nuclear localization signal motifs ensure nuclear localization required for viability. Shafer B, Chu C, Shatkin AJ Mol Cell Biol [details] 15767670 -
RAM/Fam103a1 is required for mRNA cap methylation. Gonatopoulos-Pournatzis T, Dunn S, Bounds R, Cowling VH Mol Cell [details] 22099306 -

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