Modomics - A Database of RNA Modifications

ID Card:

Full name: RNA-capping enzyme
Synonym: HCE, mRNA 5'-triphosphatase, GTP--RNA guanylyltransferase
GI: 6685628
Orf: RNGTT
COG: COG5226
UniProt: O60942
Structures: | 3S24 | 2C46 |
Alpha Fold Predicted Structure: AF-O60942-F1
Enzyme type: triphosphatase, guanylyltransferase
Position of modification - modification: m:0 - m7GpppN


PDB Structures:


3S24

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

Yet to be published

Download RCSB-PDB Structures:

Pdb Files   2C46.pdb   3S24.pdb  
Pdbx/mmCIF Files   2C46.cif   3S24.cif  


Protein sequence:

MAHNKIPPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKVKMGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPLLPDWCFEDDEDEDEDEDGKKESEPGSSASFGKRRKERLKLGAIFLEGVTVKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVTKELKQYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCTAASQGQKRKHHLDPDTELMPPPPPKRPRPLT

Comments:

Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
pppN:ppN mRNA (m) 0 9512541    9473487   
ppN:GpppN mRNA (m) 0 9512541    9473487   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M A H N K I P P R W L N C P R R G Q P V A G R F L P L K T M L G P R Y D S Q V A E E N R F H P S M L S N Y L K S L K V K M G L L V D L T N T S R F Y D R N D I E K E G I K Y I K L Q C K G H G E C P T T E N T E T F I R L C E R F N E R N P P E L I G V H C T H G F N R T G F L I C A F L V E K M D W S I E A A V A T F A Q A R P P G I Y K G D Y L K E L F R R Y G D I E E A P P P P L L P D W C F E D D E D E D E D E D G K K E S E P G S S A S F G K R R K E R L K L G A I F L E G V T V K G V T Q V T T Q P K L G E V Q Q K C H Q F C G W E G S G F P G A Q P V S M D K Q N I K L L D L K P Y K V S W K A D G T R Y M M L I D G T N E V F M I D R D N S V F H V S N L E F P F R K D L R M H L S N T L L D G E M I I D R V N G Q A V P R Y L I Y D I I K F N S Q P V G D C D F N V R L Q C I E R E I I S P R H E K M K T G L I D K T Q E P F S V R N K P F F D I C T S R K L L E G N F A K E V S H E M D G L I F Q P T G K Y K P G R C D D I L K W K P P S L N S V D F R L K I T R M G G E G L L P Q N V G L L Y V G G Y E R P F A Q I K V T K E L K Q Y D N K I I E C K F E N N S W V F M R Q R T D K S F P N A Y N T A M A V C N S I S N P V T K E M L F E F I D R C T A A S Q G Q K R K H H L D P D T E L M P P P P P K R P R P L T

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-O60942-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-O60942-F1.cif  
DSSP Secondary Structures   O60942.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Isolation and characterization of a human cDNA for mRNA 5'-capping enzyme. Yamada-Okabe T, Doi R, Shimmi O, Arisawa M, Yamada-Okabe H Nucleic Acids Res [details] 9512541 -
Cloning and characterization of two human cDNAs encoding the mRNA capping enzyme. Tsukamoto T, Shibagaki Y, Murakoshi T, Suzuki M, Nakamura A, Gotoh H, Mizumoto K Biochem Biophys Res Commun [details] 9473487 -
Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes. Pillutla RC, Yue Z, Maldonado E, Shatkin AJ J Biol Chem [details] 9705270 -
Structure of the guanylyltransferase domain of human mRNA capping enzyme. Chu C, Das K, Tyminski JR, Bauman JD, Guan R, Qiu W, Montelione GT, Arnold E, Shatkin AJ Proc Natl Acad Sci U S A [details] 21636784 -

Links:

_Wikipedia_