Modomics - A Database of RNA Modifications

ID Card:

Full name: Adenylyltransferase and sulfurtransferase UBA4
Synonym: NCS3
GI: 6321903
COG: COG0476
UniProt: P38820
Alpha Fold Predicted Structure: AF-P38820-F1
Enzyme type: adenylyltransferase, sulfurtransferase



Protein sequence:

MNDYHLEDTTSELEALRLENAQLREQLAKREDSSRDYPLSLEEYQRYGRQMIVEETGGVAGQVKLKNTKVLVVGAGGLGCPALPYLAGAGVGQIGIVDNDVVETSNLHRQVLHDSSRVGMLKCESARQYITKLNPHINVVTYPVRLNSSNAFDIFKGYNYILDCTDSPLTRYLVSDVAVNLGITVVSASGLGTEGQLTILNFNNIGPCYRCFYPTPPPPNAVTSCQEGGVIGPCIGLVGTMMAVETLKLILGIYTNENFSPFLMLYSGFPQQSLRTFKMRGRQEKCLCCGKNRTITKEAIEKGEINYELFCGARNYNVCEPDERISVDAFQRIYKDDEFLAKHIFLDVRPSHHYEISHFPEAVNIPIKNLRDMNGDLKKLQEKLPSVEKDSNIVILCRYGNDSQLATRLLKDKFGFSNVRDVRGGYFKYIDDIDQTIPKY

Comments:

Part of eukaryotic sulfur-relay system required for in 2-thiolation of mcm5S2U at tRNA wobble positions.





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M N D Y H L E D T T S E L E A L R L E N A Q L R E Q L A K R E D S S R D Y P L S L E E Y Q R Y G R Q M I V E E T G G V A G Q V K L K N T K V L V V G A G G L G C P A L P Y L A G A G V G Q I G I V D N D V V E T S N L H R Q V L H D S S R V G M L K C E S A R Q Y I T K L N P H I N V V T Y P V R L N S S N A F D I F K G Y N Y I L D C T D S P L T R Y L V S D V A V N L G I T V V S A S G L G T E G Q L T I L N F N N I G P C Y R C F Y P T P P P P N A V T S C Q E G G V I G P C I G L V G T M M A V E T L K L I L G I Y T N E N F S P F L M L Y S G F P Q Q S L R T F K M R G R Q E K C L C C G K N R T I T K E A I E K G E I N Y E L F C G A R N Y N V C E P D E R I S V D A F Q R I Y K D D E F L A K H I F L D V R P S H H Y E I S H F P E A V N I P I K N L R D M N G D L K K L Q E K L P S V E K D S N I V I L C R Y G N D S Q L A T R L L K D K F G F S N V R D V R G G Y F K Y I D D I D Q T I P K Y

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P38820-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P38820-F1.cif  





Publications:

Title Authors Journal Details PubMed Id DOI
Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. Leidel S, Pedrioli PG, Bucher T, Brost R, Costanzo M, Schmidt A, Aebersold R, Boone C, Hofmann K, Peter M Nature [details] 19145231 -
Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Noma A, Sakaguchi Y, Suzuki T Nucleic Acids Res [details] 19151091 -
A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. Huang B, Lu J, Bystrom AS RNA [details] 18755837 -
Thio-modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems. Nakai Y, Nakai M, Hayashi H J Biol Chem [details] 18664566 -