Modomics - A Database of RNA Modifications

ID Card:

Full name: Elongator complex protein 3
Synonym: HPA1, TOT3
GI: 6325171
COG: COG1243
UniProt: Q02908
Structures: | 6QK7 |
Alpha Fold Predicted Structure: AF-Q02908-F1
Complex: Elongator complex


PDB Structures:


6QK7

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

The highly conserved Elongator complex modifies transfer RNAs (tRNAs) in their wobble base position, thereby regulating protein synthesis and ensuring proteome stability. The precise mechanisms of tRNA recognition and its modification reaction remain elusive. Here, we show cryo-electron microscopy structures of the catalytic subcomplex of Elongator and its tRNA-bound state at resolutions of 3.3 and 4.4 Å. The structures resolve details of the catalytic site, including the substrate tRNA, the iron-sulfur cluster, and a SAM molecule, which are all validated by mutational analyses in vitro and in vivo. tRNA binding induces conformational rearrangements, which precisely position the targeted anticodon base in the active site. Our results provide the molecular basis for substrate recognition of Elongator, essential to understand its cellular function and role in neurodegenerative diseases and cancer.

Download RCSB-PDB Structures:

Pdb Files   6QK7.pdb  
Pdbx/mmCIF Files   6QK7.cif  


Protein sequence:

MARHGKGPKTNKKKLAPEKERFIQCCADITLELTDSLTSGTTREINLNGLITKYSKKYKLKQQPRLTDIINSIPDQYKKYLLPKLKAKPVRTASGIAVVAVMCKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRVEQLKQLGHSIDKVEYVLMGGTFMSLPKEYREDFIVKLHNALSGFNGNDIDEAILYSQQSLTKCVGITIETRPDYCTQTHLDDMLKYGCTRLEIGVQSLYEDVARDTNRGHTVRSVCETFAVSKDAGYKVVSHMMPDLPNVGMERDIEQFKEYFENPDFRTDGLKIYPTLVIRGTGLYELWKTGRYKSYSANALVDLVARILALVPPWTRIYRVQRDIPMPLVTSGVDNGNLRELALARMKDLGTTCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANGGWETFLSYEDPKKDILIGLLRLRKASKKYTYRKEFTSQRTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAERIAKEEHGSEKISVISGVGVRNYYGKLGYELDGPYMSKRI

Comments:

One of the six protein cofactors required for the synthesis of 5-carboxymethyl group (cm5) on the wobble uridine-34 of a few tRNA. Acetate or acetyl-CoA is the donor of acetyl group, but the detailed mechanism of the reaction is still unknown. The cm5U derivative is the intermediate for further biochemical transformation of U34 derivative to either 5-methoxycarbonylmethyl uridine (mcm5U) catalysed by Trm9/Trm112 or 5-carbamoylmethyluridine (ncm5U) catalysed by a still unknown enzyme. Mutation in Elp (especially Elp3) influences telomeric gene silencing and DNA damage response. The multi-subunit complex El1-6 also interacts with elongating RNA polymerase II (RNAPII) is thought to facilitate transcription through histone acetylation.





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M A R H G K G P K T N K K K L A P E K E R F I Q C C A D I T L E L T D S L T S G T T R E I N L N G L I T K Y S K K Y K L K Q Q P R L T D I I N S I P D Q Y K K Y L L P K L K A K P V R T A S G I A V V A V M C K P H R C P H I A Y T G N I C V Y C P G G P D S D F E Y S T Q S Y T G Y E P T S M R A I R A R Y D P Y E Q A R G R V E Q L K Q L G H S I D K V E Y V L M G G T F M S L P K E Y R E D F I V K L H N A L S G F N G N D I D E A I L Y S Q Q S L T K C V G I T I E T R P D Y C T Q T H L D D M L K Y G C T R L E I G V Q S L Y E D V A R D T N R G H T V R S V C E T F A V S K D A G Y K V V S H M M P D L P N V G M E R D I E Q F K E Y F E N P D F R T D G L K I Y P T L V I R G T G L Y E L W K T G R Y K S Y S A N A L V D L V A R I L A L V P P W T R I Y R V Q R D I P M P L V T S G V D N G N L R E L A L A R M K D L G T T C R D V R T R E V G I Q E V H H K V Q P D Q V E L I R R D Y Y A N G G W E T F L S Y E D P K K D I L I G L L R L R K A S K K Y T Y R K E F T S Q R T S I V R E L H V Y G S V V P L H S R D P R K F Q H Q G F G T L L M E E A E R I A K E E H G S E K I S V I S G V G V R N Y Y G K L G Y E L D G P Y M S K R I

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q02908-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q02908-F1.cif  
DSSP Secondary Structures   Q02908.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
A conserved modified wobble nucleoside (mcm5s2U) in lysyl-tRNA is required for viability in yeast. Bjork GR, Huang B, Persson OP, Bystrom AS RNA [details] 17592039 -
A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. Huang B, Lu J, Bystrom AS RNA [details] 18755837 -
An early step in wobble uridine tRNA modification requires the Elongator complex. Huang B, Johansson MJ, Bystrom AS RNA [details] 15769872 -
Elongator complex influences telomeric gene silencing and DNA damage response by its role in wobble uridine tRNA modification. Chen C, Huang B, Eliasson M, Ryden P, Bystrom AS... PLoS Genet [details] 21912530 -