Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA (guanine(6)-N2)-methyltransferase
Synonym: Trm14, MJ0438
GI: 15668614
COG: COG0116
UniProt: Q57880
Alpha Fold Predicted Structure: AF-Q57880-F1
Enzyme type: methyltransferase
Position of modification - modification: t:6 - m2G



Protein sequence:

MDYYVTLSPGLEKISKNEIESFGGKIKEIRENKGRIFFSGDLKLIPKINYLSRTIERMNILLHREEIPNIALDDIYKRVYNIDWTEWIKENQSFAIRPLRAGEHNFTSIDIGRVAGEAVIKSYQRDKNIRLKVNLDEPDVIVRVEVIFDELIVGIDTTGDIALDKRGYRVFNHPAHLNATIASSLVYLSDWKDDEMLLDPMCGSGTIPIEGALMKRNIPPGKFRENKYGFKFIDIFGYELLDKIKKEIVENKNIYKIIGLDKNQKYLDGAKDNAKNAEVLDTIEFICGDATKLHEKFNESDVIIANPPYGIRIGSKRSVKKLYDEFLSSAKEIMHGSSRLIVITAEDKMFKDAIAKNNFEVKEEFNVMFGGLMTRVFYLTL

Comments:

Distant homologs of Trm14 were also identified in eykaryota known to possess the m2G6 tRNA modification




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
G:m2G RNA tRNA 6 GCA GCA tRNACysGCA acceptor-stem Prokaryotic Cytosol 21693558   

Alpha Fold Predicted Structure:






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Protein sequence:

M D Y Y V T L S P G L E K I S K N E I E S F G G K I K E I R E N K G R I F F S G D L K L I P K I N Y L S R T I E R M N I L L H R E E I P N I A L D D I Y K R V Y N I D W T E W I K E N Q S F A I R P L R A G E H N F T S I D I G R V A G E A V I K S Y Q R D K N I R L K V N L D E P D V I V R V E V I F D E L I V G I D T T G D I A L D K R G Y R V F N H P A H L N A T I A S S L V Y L S D W K D D E M L L D P M C G S G T I P I E G A L M K R N I P P G K F R E N K Y G F K F I D I F G Y E L L D K I K K E I V E N K N I Y K I I G L D K N Q K Y L D G A K D N A K N A E V L D T I E F I C G D A T K L H E K F N E S D V I I A N P P Y G I R I G S K R S V K K L Y D E F L S S A K E I M H G S S R L I V I T A E D K M F K D A I A K N N F E V K E E F N V M F G G L M T R V F Y L T L

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q57880-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q57880-F1.cif  
DSSP Secondary Structures   Q57880.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Formation of m2G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14. Menezes S, Gaston KW, Krivos KL, Apolinario EE, Reich NO, Sowers KR, Limbach PA, Perona JJ Nucleic Acids Res [details] 21693558 -