Modomics - A Database of RNA Modifications

ID Card:

Full name: Ribosomal RNA small subunit methyltransferase G
Synonym: gidB
GI: 121190
COG: COG0357
UniProt: P25813
Structures: | 1XDZ |
Alpha Fold Predicted Structure: AF-P25813-F1
Enzyme type: methyltransferase
Position of modification - modification: s:533(527) - m7G


PDB Structures:


1XDZ

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:



Download RCSB-PDB Structures:

Pdb Files   1XDZ.pdb  
Pdbx/mmCIF Files   1XDZ.cif  


Protein sequence:

MNIEEFTSGLAEKGISLSPRQLEQFELYYDMLVEWNEKINLTSITEKKEVYLKHFYDSITAAFYVDFNQVNTICDVGAGAGFPSLPIKICFPHLHVTIVDSLNKRITFLEKLSEALQLENTTFCHDRAETFGQRKDVRESYDIVTARAVARLSVLSELCLPLVKKNGLFVALKAASAEEELNAGKKAITTLGGELENIHSFKLPIEESDRNIMVIRKIKNTPKKYPRKPGTPNKSPIEG

Comments:

RsmG is responsible for the synthesis of m7G535 (position 527 in E. coli) in the universally conserved loop of hairpin 18 in domain I. This modified nucleotide plays an essential role in binding antibiotics like streptomycin and kasugamycin. However the dependence on RsmG for antibiotic binding depends on the activity of RsmA (KsgA) catalyzing the formation of m6,6A1518,1519 at the 3'-end of 16S rRNA by RsmA (KsgA). In B. subtilis, mutations in both RsmA and RsmG allow better cell viability in the presence of antibiotics. AdoMet is the methyl group donor.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
G:m7G RNA rRNA 535 LSU-16S Prokaryotic Cytosol 17573471   

Alpha Fold Predicted Structure:






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Protein sequence:

M N I E E F T S G L A E K G I S L S P R Q L E Q F E L Y Y D M L V E W N E K I N L T S I T E K K E V Y L K H F Y D S I T A A F Y V D F N Q V N T I C D V G A G A G F P S L P I K I C F P H L H V T I V D S L N K R I T F L E K L S E A L Q L E N T T F C H D R A E T F G Q R K D V R E S Y D I V T A R A V A R L S V L S E L C L P L V K K N G L F V A L K A A S A E E E L N A G K K A I T T L G G E L E N I H S F K L P I E E S D R N I M V I R K I K N T P K K Y P R K P G T P N K S P I E G

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P25813-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P25813-F1.cif  
DSSP Secondary Structures   P25813.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants. Nishimura K, Johansen SK, Inaoka T, Hosaka T, Tokuyama S, Tahara Y, Okamoto S, Kawamura F, Douthwaite S, Ochi K J Bacteriol [details] 17573471 -
Inactivation of KsgA, a 16S rRNA methyltransferase, causes vigorous emergence of mutants with high-level kasugamycin resistance. Ochi K, Kim JY, Tanaka Y, Wang G, Masuda K, Nanamiya H, Okamoto S, Tokuyama S, Adachi Y, Kawamura F Antimicrob Agents Chemother [details] 19001112 -