Modomics - A Database of RNA Modifications

ID Card:

Full name: Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB
Synonym: YqeV
GI: 1730990
Orf: BSU25430
COG: COG0621
UniProt: P54462
Alpha Fold Predicted Structure: AF-P54462-F1
Enzyme type: methyltiotransferase
Position of modification - modification: t:37 - ms2t6A



Protein sequence:

MATVAFHTLGCKVNHYETEAIWQLFKEAGYERRDFEQTADVYVINTCTVTNTGDKKSRQVIRRAIRQNPDGVICVTGCYAQTSPAEIMAIPGVDIVVGTQDREKMLGYIDQYREERQPINGVSNIMKARVYEELDVPAFTDRTRASLKIQEGCNNFCTFCIIPWARGLLRSRDPEEVIKQAQQLVDAGYKEIVLTGIHTGGYGEDMKDYNFAKLLSELDTRVEGVKRIRISSIEASQITDEVIEVLDRSDKIVNHLHIPIQSGSNTVLKRMRRKYTMEFFADRLNKLKKALPGLAVTSDVIVGFPGETEEEFMETYNFIKEHKFSELHVFPYSKRTGTPAARMEDQVDENVKNERVHRLIALSDQLAKEYASQYENEVLEIIPEEAFKETEEENMFVGYTDNYMKVVFKGTEDMIGKIVKVKILKAGYPYNEGQFVRVVEDEITEHMRLSS

Comments:

ms2 on t6A has been identified so far only in bacterial U36A37-containing tRNALys (anticodon U*UU, U* stands for a modified U-residue). The B. subtilis TmtB (Yqev) is a radical-SAM methyltransferase that displays two [4Fe-4S] clusters and one TRAM-domain. However, this characteristic tRNA binding motif is not involved in the discrimination of t6A37 or i6A37. A distinct methylthiotransferase (MiaB) is acting on i6A-containing tRNA, and another one (RimO) is acting exclusively on ribosomal protein S12.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
t6A:ms2t6A RNA tRNA 37 GGT GGT tRNAThrGGT anticodon-loop Prokaryotic Cytosol 20584901   
t6A:ms2t6A RNA tRNA 37 TGT TGT tRNAThrTGT anticodon-loop Prokaryotic Cytosol 20584901   
t6A:ms2t6A RNA tRNA 37 GCT GCT tRNASerGCT anticodon-loop Prokaryotic Cytosol 20584901   
t6A:ms2t6A RNA tRNA 37 CCT CCT tRNAArgCCT anticodon-loop Prokaryotic Cytosol 20584901   
t6A:ms2t6A RNA tRNA 37 TCT TCT tRNAArgTCT anticodon-loop Prokaryotic Cytosol 20584901   
t6A:ms2t6A RNA tRNA 37 GTT GTT tRNAAsnGTT anticodon-loop Prokaryotic Cytosol 20584901   
t6A:ms2t6A RNA tRNA 37 TTT TTT tRNALysTTT anticodon-loop Prokaryotic Cytosol 20584901   
t6A:ms2t6A RNA tRNA 37 GAT GAT tRNAIleGAT anticodon-loop Prokaryotic Cytosol 20584901   
t6A:ms2t6A RNA tRNA 37 CAT CAT tRNAIleCAT anticodon-loop Prokaryotic Cytosol 20584901   
t6A:ms2t6A RNA tRNA 37 CAT CAT tRNAMet/fMetCAT anticodon-loop Prokaryotic Cytosol 20584901   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M A T V A F H T L G C K V N H Y E T E A I W Q L F K E A G Y E R R D F E Q T A D V Y V I N T C T V T N T G D K K S R Q V I R R A I R Q N P D G V I C V T G C Y A Q T S P A E I M A I P G V D I V V G T Q D R E K M L G Y I D Q Y R E E R Q P I N G V S N I M K A R V Y E E L D V P A F T D R T R A S L K I Q E G C N N F C T F C I I P W A R G L L R S R D P E E V I K Q A Q Q L V D A G Y K E I V L T G I H T G G Y G E D M K D Y N F A K L L S E L D T R V E G V K R I R I S S I E A S Q I T D E V I E V L D R S D K I V N H L H I P I Q S G S N T V L K R M R R K Y T M E F F A D R L N K L K K A L P G L A V T S D V I V G F P G E T E E E F M E T Y N F I K E H K F S E L H V F P Y S K R T G T P A A R M E D Q V D E N V K N E R V H R L I A L S D Q L A K E Y A S Q Y E N E V L E I I P E E A F K E T E E E N M F V G Y T D N Y M K V V F K G T E D M I G K I V K V K I L K A G Y P Y N E G Q F V R V V E D E I T E H M R L S S

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P54462-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P54462-F1.cif  
DSSP Secondary Structures   P54462.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis. Anton BP, Russell SP, Vertrees J, Kasif S, Raleigh EA, Limbach PA, Roberts RJ Nucleic Acids Res [details] 20472640 -
Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA. Arragain S, Handelman SK, Forouhar F, Wei FY, Tomizawa K, Hunt JF, Douki T, Fontecave M, Mulliez E, Atta M J Biol Chem [details] 20584901 -
The methylthiolation reaction mediated by the Radical-SAM enzymes. Atta M, Arragain S, Fontecave M, Mulliez E, Hunt JF, Luff JD, Forouhar F Biochim Biophys Acta [details] 22178611 -

Links:

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