Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA dimethylallyltransferase 2
Synonym: IPT2, At2g27760
GI: 6006718
Orf: F15K20
COG: COG0324
UniProt: Q9ZUX7
Alpha Fold Predicted Structure: AF-Q9ZUX7-F1
Enzyme type: dimethylallyltransferase predicted
Position of modification - modification: t:37 - i6A



Protein sequence:

MMMLNPSNGGIEGEKMKKKAKVVVIMGPTGSGKSKLAVDLASHFPVEIINADAMQIYSGLDVLTNKVTVDEQKGVPHHLLGTVSSDMEFTARDFRDFTVPLIEEIVSRNHIPVLVGGTHYYIQAVVSKFLLDDAAEDTEECCADVASVVDQDMVVESVFGRDDLSHGYELLKELDPVAANRIHPNNHRKINQYLSLHASRGVLPSKLYQGKTAENWGCINASRFDYCLICMDAETAVLDRYVEQRVDAMVDAGLLDEVYDIYKPGADYTRGLRQSIGVREFEDFLKIHLSETCAGHLTSLSNDDKVMKENLRKILNFPKDDKLRIMLEEAIDRVKLNTRRLLRRQKRRVSRLETVFGWNIHYIDATEYILSKSEESWNAQVVKPASEIIRCFLETETESGRDPTSGKSIERDLWTQYVCEACGNKILRGRHEWEHHKQGRTHRKRTTRHKNSQTYKNREVQEAEVN

Comments:

This RNA modification enzyme is also involved in the cis-type cytokinin biosynthesis. Arabidopsis thaliana derived gene was shown to complement S. cerevisiae mutant lacking Mod5.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
A:i6A tRNA (t) many/many/mitochondrion 37 11999372   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M M M L N P S N G G I E G E K M K K K A K V V V I M G P T G S G K S K L A V D L A S H F P V E I I N A D A M Q I Y S G L D V L T N K V T V D E Q K G V P H H L L G T V S S D M E F T A R D F R D F T V P L I E E I V S R N H I P V L V G G T H Y Y I Q A V V S K F L L D D A A E D T E E C C A D V A S V V D Q D M V V E S V F G R D D L S H G Y E L L K E L D P V A A N R I H P N N H R K I N Q Y L S L H A S R G V L P S K L Y Q G K T A E N W G C I N A S R F D Y C L I C M D A E T A V L D R Y V E Q R V D A M V D A G L L D E V Y D I Y K P G A D Y T R G L R Q S I G V R E F E D F L K I H L S E T C A G H L T S L S N D D K V M K E N L R K I L N F P K D D K L R I M L E E A I D R V K L N T R R L L R R Q K R R V S R L E T V F G W N I H Y I D A T E Y I L S K S E E S W N A Q V V K P A S E I I R C F L E T E T E S G R D P T S G K S I E R D L W T Q Y V C E A C G N K I L R G R H E W E H H K Q G R T H R K R T T R H K N S Q T Y K N R E V Q E A E V N

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q9ZUX7-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q9ZUX7-F1.cif  
DSSP Secondary Structures   Q9ZUX7.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Identification of a tRNA isopentenyltransferase gene from Arabidopsis thaliana. Golovko A, Sitbon F, Tillberg E, Nicander B Plant Mol Biol [details] 11999372 -
Roles of Arabidopsis ATP/ADP isopentenyltransferases and tRNA isopentenyltransferases in cytokinin biosynthesis. Miyawaki K, Tarkowski P, Matsumoto-Kitano M, Kato T, Sato S, Tarkowska D, Tabata S, Sandberg G, Kakimoto T Proc Natl Acad Sci U S A [details] 17062755 -

Links:

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