Modomics - A Database of RNA Modifications

ID Card:

Full name: Regulatory protein Cgi121
Synonym: MJ0187
GI: 15668359
COG: COG1617
UniProt: Q57646
Structures: | 2K8Y | 3ENH |
Alpha Fold Predicted Structure: AF-Q57646-F1
Complex: EKC/KEOPS
Enzyme type: pseudouridine synthase
Position of modification - modification: t:37 - t6A


PDB Structures:


2K8Y

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

Kae1 is a universally conserved ATPase and part of the essential gene set in bacteria. In archaea and eukaryotes, Kae1 is embedded within the protein kinase-containing KEOPS complex. Mutation of KEOPS subunits in yeast leads to striking telomere and transcription defects, but the exact biochemical function of KEOPS is not known. As a first step to elucidating its function, we solved the atomic structure of archaea-derived KEOPS complexes involving Kae1, Bud32, Pcc1, and Cgi121 subunits. Our studies suggest that Kae1 is regulated at two levels by the primordial protein kinase Bud32, which is itself regulated by Cgi121. Moreover, Pcc1 appears to function as a dimerization module, perhaps suggesting that KEOPS may be a processive molecular machine. Lastly, as Bud32 lacks the conventional substrate-recognition infrastructure of eukaryotic protein kinases including an activation segment, Bud32 may provide a glimpse of the evolutionary history of the protein kinase family.

Download RCSB-PDB Structures:

Pdb Files   2K8Y.pdb   3ENH.pdb  
Pdbx/mmCIF Files   2K8Y.cif   3ENH.cif  


Protein sequence:

MIIRGIRGARINNEIFNLGLKFQILNADVVATKKHVLHAINQAKTKKPIAKSFWMEILVRASGQRQIHEAIKIIGAKDGNVCLICEDEETFRKIYELIGGEIDDSVLEINEDKERLIREIFKIRGFGNVVERVLEKIALIELKKE

Comments:

Absent in bacteria, present only in eykaryota and archaea. Works with other proteins of the KEC/KEOPS complex Bud32, Pcc1 and possibly Gon7. It is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, a step in the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Cgi121 stimulates Bud32 kinase activity via an activation of Bud32 autophosphorylation.





Alpha Fold Predicted Structure:






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Protein sequence:

M I I R G I R G A R I N N E I F N L G L K F Q I L N A D V V A T K K H V L H A I N Q A K T K K P I A K S F W M E I L V R A S G Q R Q I H E A I K I I G A K D G N V C L I C E D E E T F R K I Y E L I G G E I D D S V L E I N E D K E R L I R E I F K I R G F G N V V E R V L E K I A L I E L K K E

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q57646-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q57646-F1.cif  
DSSP Secondary Structures   Q57646.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine. Mao DY, Neculai D, Downey M, Orlicky S, Haffani YZ, Ceccarelli DF, Ho JS, Szilard RK, Zhang W, Ho CS, Wan L, Fares C, Rumpel S, Kurinov I, Arrowsmith CH, Durocher D, Sicheri F Mol Cell [details] 18951093 -

Links:

_PubMed_