Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA(pseudouridine54-N1)-methyltransferase
Synonym: HVO_1989
GI: 292656118
COG: COG1901
UniProt: D4GTL8
Structures: | |
Alpha Fold Predicted Structure: AF-D4GTL8-F1
Enzyme type: methyltransferase
Position of modification - modification: t:54 - m1Y



Protein sequence:

MRQFIVTGHDAPTTPDFSLDDIAGGAGRLDVLCRCVNSAFFLSHDIREDVRVHLVLGDEYTVRFEGSELRRLNPDERSTAALIRKALEKREEAIGHMPAESSPGVSIRRMGFETTLEEAASDATVVELHEDGDPVVQVEPPENPLFVLSDHHDFTDEEAELLAAAADERVRLGPEILHADHSITVAHNYLDTAGYSRY

Comments:

AdoMet dependent methyltransferase. Pseudouridine in position 55 is important for efficient conversion of Psi54 to m1Psi54. This modification is located in the T-loop and is important for the tertiary structure of the tRNA molecule. Crystal structure of TrmY from M. jannaschii was solved at 1.4 and 1.55A resolution (see the entry for M. jannaschii TrmY).





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M R Q F I V T G H D A P T T P D F S L D D I A G G A G R L D V L C R C V N S A F F L S H D I R E D V R V H L V L G D E Y T V R F E G S E L R R L N P D E R S T A A L I R K A L E K R E E A I G H M P A E S S P G V S I R R M G F E T T L E E A A S D A T V V E L H E D G D P V V Q V E P P E N P L F V L S D H H D F T D E E A E L L A A A A D E R V R L G P E I L H A D H S I T V A H N Y L D T A G Y S R Y
20406080100120140160180MRQFIVTGHDAPTTPDFSLDDIAGGAGRLDVLCRCVNSAFFLSHDIREDVRVHLVLGDEYTVRFEGSELRRLNPDERSTAALIRKALEKREEAIGHMPAESSPGVSIRRMGFETTLEEAASDATVVELHEDGDPVVQVEPPENPLFVLSDHHDFTDEEAELLAAAADERVRLGPEILHADHSITVAHNYLDTAGYSRYSequenceGHTSN

Enter the variants

Position

Original

Variant

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-D4GTL8-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-D4GTL8-F1.cif  
DSSP Secondary Structures   D4GTL8.dssp  





Publications:

Links:

_PubMed_
_HaloferaxWiki_