Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA-specific adenosine deaminase, chloroplastic
Synonym: AtTadA
GI: 15221490
Orf: F14K14.18, F24J5.5
COG: COG0590
UniProt: Q9S7I0
Structures: | |
Alpha Fold Predicted Structure: AF-Q9S7I0-F1
Enzyme type: deaminase
Position of modification - modification: t:34 - I



Protein sequence:

MFNTYTNSLQWPIRSRNQQDYCSLLPERSESYKLSKAYTSSRCYCVSSRSSCCCCCSTPSSSSFVKPKVLINPGFVLYGVRQSTLIQWPSFQRRLLVGGGRLMGCEVYSSCDGIRRKNRSFKLRCLEESDECCGGRSCSDDVEAMISFLSEELIDEERKWNLVSRVKEKKKVGNVRKVSVEGSNSYGNGRVSQRVKKPEGFGRRKEIKEDVKLNERYDCEHCGRRKKSSELESESRRGSKLVTGEYIGKSYRGDEEREVRPRRRKSSSCSSYYSLASSGEFESDTEDQEEDVEIYRENVRSSEKKVVDQSAKRLKSRKEASQMHSRKKRDESSTGVDSRYQKQIFEEGENSNQAVTLNQRRRKKFSQTENRVSESTGNYEEDMEIHEVHVNDAETSSQNQKLFNEREDYRVHSIRNDSGNENIESSQHQLKERLETRYSSEDRVSEMRRRTKYSSSQEEGINVLQNFPEVTNNQQPLVEERISKQAGTRRTTEHISESSEIHDIDIRNTYVSQREDQIRNQEVHAGLVSGLQSERKQQDYHIEHNPLQTTQSDRTSVSVSHTSDAVRYTEIQRKSEKRLIGQGSTTAVQSDSKVEKNGAQKEDSRLDHANSKKDGQTTLGLQSYQSKLSEEASSSQSSLMASRTKLQLVDLVSEEMQGSETTLIPPSSQLVSRRSGQSYRTGGVSIQEISHGTSESGYTTAFEHPRAGASVNSQSAGELMGFTSHEDAMGSAHRLEQASEKYVGEFVKKAKHGVINPETEEQRAESNQLKRRDSRRSSGGSGAKGPSDEMWVTDSAQGTPHPGATEGNAAVGNAIFKRNGRSLWNVIADIARLRWGSRAGSPDSSAKPAGRSSPNESVSSATWFSGREHDGSSDDNTKGDKVLPQEAPSLHQVEVGQTSPRSQSEYPGTTKLKQRSERHEGVVSSPSSTILEGGSVSNRMSSTSGNQIVGVDEEEGGNFEFRLPETALTEVPMKLPSRNLIRSPPIKESSESSLTEASSDQNFTVGEGRRYPRMDAGQNPLLFPGRNLRSPAVMEPPVPRPRMVSGSSSLREQVEQQQPLSAKSQEETGSVSADSALIQRKLQRNKQVVRDSFEEWEEAYKVEAERRTVDEIFMREALVEAKKAADTWEVPVGAVLVHDGKIIARGYNLVEELRDSTAHAEMICIREGSKALRSWRLADTTLYVTLEPCPMCAGAILQARVNTLVWGAPNKLLGADGSWIRLFPGGEGNGSEASEKPPPPVHPFHPKMTIRRGVLESECAQTMQQFFQLRRKKKDKNSDPPTPTDHHHHHLPKLLNKMHQVLPFFCL

Comments:

A-to-I tRNA editing enzyme from chloroplasts. This enzyme has an unusual structure in that it harbors a large N-terminal domain of more than 1000 amino acids long which is not required for catalytic activity. Only the C-terminal region displays sequence similarity to the tRNA adenosine deaminase TadA from Escherichia coli. Moreover, AtTadA is imported into the chloroplast in vivo.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
A:I RNA tRNA 34 ACG ICG tRNAArgICG Plastid 19460869   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M F N T Y T N S L Q W P I R S R N Q Q D Y C S L L P E R S E S Y K L S K A Y T S S R C Y C V S S R S S C C C C C S T P S S S S F V K P K V L I N P G F V L Y G V R Q S T L I Q W P S F Q R R L L V G G G R L M G C E V Y S S C D G I R R K N R S F K L R C L E E S D E C C G G R S C S D D V E A M I S F L S E E L I D E E R K W N L V S R V K E K K K V G N V R K V S V E G S N S Y G N G R V S Q R V K K P E G F G R R K E I K E D V K L N E R Y D C E H C G R R K K S S E L E S E S R R G S K L V T G E Y I G K S Y R G D E E R E V R P R R R K S S S C S S Y Y S L A S S G E F E S D T E D Q E E D V E I Y R E N V R S S E K K V V D Q S A K R L K S R K E A S Q M H S R K K R D E S S T G V D S R Y Q K Q I F E E G E N S N Q A V T L N Q R R R K K F S Q T E N R V S E S T G N Y E E D M E I H E V H V N D A E T S S Q N Q K L F N E R E D Y R V H S I R N D S G N E N I E S S Q H Q L K E R L E T R Y S S E D R V S E M R R R T K Y S S S Q E E G I N V L Q N F P E V T N N Q Q P L V E E R I S K Q A G T R R T T E H I S E S S E I H D I D I R N T Y V S Q R E D Q I R N Q E V H A G L V S G L Q S E R K Q Q D Y H I E H N P L Q T T Q S D R T S V S V S H T S D A V R Y T E I Q R K S E K R L I G Q G S T T A V Q S D S K V E K N G A Q K E D S R L D H A N S K K D G Q T T L G L Q S Y Q S K L S E E A S S S Q S S L M A S R T K L Q L V D L V S E E M Q G S E T T L I P P S S Q L V S R R S G Q S Y R T G G V S I Q E I S H G T S E S G Y T T A F E H P R A G A S V N S Q S A G E L M G F T S H E D A M G S A H R L E Q A S E K Y V G E F V K K A K H G V I N P E T E E Q R A E S N Q L K R R D S R R S S G G S G A K G P S D E M W V T D S A Q G T P H P G A T E G N A A V G N A I F K R N G R S L W N V I A D I A R L R W G S R A G S P D S S A K P A G R S S P N E S V S S A T W F S G R E H D G S S D D N T K G D K V L P Q E A P S L H Q V E V G Q T S P R S Q S E Y P G T T K L K Q R S E R H E G V V S S P S S T I L E G G S V S N R M S S T S G N Q I V G V D E E E G G N F E F R L P E T A L T E V P M K L P S R N L I R S P P I K E S S E S S L T E A S S D Q N F T V G E G R R Y P R M D A G Q N P L L F P G R N L R S P A V M E P P V P R P R M V S G S S S L R E Q V E Q Q Q P L S A K S Q E E T G S V S A D S A L I Q R K L Q R N K Q V V R D S F E E W E E A Y K V E A E R R T V D E I F M R E A L V E A K K A A D T W E V P V G A V L V H D G K I I A R G Y N L V E E L R D S T A H A E M I C I R E G S K A L R S W R L A D T T L Y V T L E P C P M C A G A I L Q A R V N T L V W G A P N K L L G A D G S W I R L F P G G E G N G S E A S E K P P P P V H P F H P K M T I R R G V L E S E C A Q T M Q Q F F Q L R R K K K D K N S D P P T P T D H H H H H L P K L L N K M H Q V L P F F C L

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q9S7I0-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q9S7I0-F1.cif  
DSSP Secondary Structures   Q9S7I0.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Identification of the chloroplast adenosine-to-inosine tRNA editing enzyme. Karcher D, Bock R RNA [details] 19460869 -
Arabidopsis tRNA adenosine deaminase arginine edits the wobble nucleotide of chloroplast tRNAArg(ACG) and is essential for efficient chloroplast translation. Delannoy E, Le Ret M, Faivre-Nitschke E, Estavillo GM, Bergdoll M, Taylor NL, Pogson BJ, Small I, Imbault P, Gualberto JM Plant Cell [details] 19602623 -
A-to-I and C-to-U editing within transfer RNAs. Su AA, Randau L Biochemistry (Mosc) [details] 22022967 -
Determinants of tRNA editing and modification: Avoiding conundrums, affecting function. Paris Z, Fleming IM, Alfonzo JD Semin Cell Dev Biol [details] 22024020 -

Links:

_Wikipedia - RNA editing_
_Wikipedia - Deamination_
_TAIR_