Modomics - A Database of RNA Modifications

ID Card:

Full name: 50S ribosomal protein L7Ae
Synonym: rpl7ae, MJ1203
GI: 15669389
COG: COG1358
UniProt: P54066
Structures: | 1RA4 | 1XBI | 1SDS |
Alpha Fold Predicted Structure: AF-P54066-F1
Complex: C/D RNP


PDB Structures:


1RA4

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary conservation, functional characteristics, and structural details have been analyzed. Comparison of the L7Ae structure to those of a number of related proteins with diverse functions has revealed significant structural homology which suggests that this protein fold is an ancient RNA-binding motif. Notably, the free M. jannaschii L7Ae structure is essentially identical to that with RNA bound, suggesting that RNA binding occurs through an induced-fit interaction. Circular dichroism experiments show that box C/D and C'/D' RNA motifs undergo conformational changes when magnesium or the L7Ae protein is added, corroborating the induced-fit model for L7Ae-box C/D RNA interactions.

Download RCSB-PDB Structures:

Pdb Files   1RA4.pdb   1SDS.pdb   1XBI.pdb  
Pdbx/mmCIF Files   1RA4.cif   1SDS.cif   1XBI.cif  


Protein sequence:

MAVYVKFKVPEEIQKELLDAVAKAQKIKKGANEVTKAVERGIAKLVIIAEDVKPEEVVAHLPYLCEEKGIPYAYVASKQDLGKAAGLEVAASSVAIINEGDAEELKVLIEKVNVLKQ

Comments:

L7Ae protein, together with fused Nop56/58 (also designated Nop5) and Fibrillarin methyltranferase (FlpA), makes the core of C/D RNP complex in Archaea. It is a homologue of S. cerevisiae Snu13 and mammalian 15.5kD protein. Archaeal L7Ae is also a subunit of H/ACA RNP complex, a subunit of RNase P and one of the ribosomal protein of the 50S subunit. In all cases L7Ae binds to a common kink-turn motif of RNA.





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M A V Y V K F K V P E E I Q K E L L D A V A K A Q K I K K G A N E V T K A V E R G I A K L V I I A E D V K P E E V V A H L P Y L C E E K G I P Y A Y V A S K Q D L G K A A G L E V A A S S V A I I N E G D A E E L K V L I E K V N V L K Q

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P54066-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P54066-F1.cif  
DSSP Secondary Structures   P54066.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs. Suryadi J, Tran EJ, Maxwell ES, Brown BA 2nd Biochemistry [details] 16008351 -
Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA at 1.8 A resolution. Hamma T, Ferre-D'Amare AR Structure [details] 15130481 -

Links:

_PubMed_