Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA (adenosine(37)-N6)-methyltransferase
Synonym: YfiC, TrmN6
GI: 13638617
COG: COG4123
UniProt: P31825
Alpha Fold Predicted Structure: AF-P31825-F1
Enzyme type: methyltransferase
Position of modification - modification: t:37 - m6A



Protein sequence:

MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQAFYLFM

Comments:

SAM-dependent tRNA (adenine-N(6)-)-methyltransferase; modifies A37 of tRNA1Val(cmo5UAC).




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
A:m6A RNA tRNA 37 VAC VAC tRNAValVAC anticodon Prokaryotic Cytosol 19383770   

Alpha Fold Predicted Structure:






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Protein sequence:

M S Q S T S V L R R N G F T F K Q F F V A H D R C A M K V G T D G I L L G A W A P V A G V K R C L D I G A G S G L L A L M L A Q R T D D S V M I D A V E L E S E A A A Q A Q E N I N Q S P W A E R I N V H T A D I Q Q W I T Q Q T V R F D L I I S N P P Y Y Q Q G V E C S T P Q R E Q A R Y T T T L D H P S L L T C A A E C I T E E G F F C V V L P E Q I G N G F T E L A L S M G W H L R L R T D V A E N E A R L P H R V L L A F S P Q A G E C F S D R L V I R G P D Q N Y S E A Y T A L T Q A F Y L F M

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P31825-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P31825-F1.cif  
DSSP Secondary Structures   P31825.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
The yfiC gene of E. coli encodes an adenine-N6 methyltransferase that specifically modifies A37 of tRNA1Val(cmo5UAC). Golovina AY, Sergiev PV, Golovin AV, Serebryakova MV, Demina I, Govorun VM, Dontsova OA RNA [details] 19383770 -