Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA guanosine(34) transglycosylase
Synonym: JW0396
GI: 67473024
Orf: b0406
COG: COG0343
UniProt: P0A847
Alpha Fold Predicted Structure: AF-P0A847-F1
Enzyme type: transglycosylase
Position of modification - modification: t:34 - Q



Protein sequence:

MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD

Comments:

Reaction preQ0(b):preQ0 do not occur under physiological conditions, because normally all preQ0(b) is converted to preQ1(b) by QueF. Do not exist in S. cerevisiae, exist in other yeast (Tgt, O94460, GI20514973, SPAC1687.19c). Structure in complex with tRNA was solved for Tgt enzyme from Zymomonas mobilis (1Q2R).




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
preQ1base:preQ1 tRNA (t)   34

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M K F E L D T T D G R A R R G R L V F D R G V V E T P C F M P V G T Y G T V K G M T P E E V E A T G A Q I I L G N T F H L W L R P G Q E I M K L H G D L H D F M Q W K G P I L T D S G G F Q V F S L G D I R K I T E Q G V H F R N P I N G D P I F L D P E K S M E I Q Y D L G S D I V M I F D E C T P Y P A D W D Y A K R S M E M S L R W A K R S R E R F D S L G N K N A L F G I I Q G S V Y E D L R D I S V K G L V D I G F D G Y A V G G L A V G E P K A D M H R I L E H V C P Q I P A D K P R Y L M G V G K P E D L V E G V R R G I D M F D C V M P T R N A R N G H L F V T D G V V K I R N A K Y K S D T G P L D P E C D C Y T C R N Y S R A Y L H H L D R C N E I L G A R L N T I H N L R Y Y Q R L M A G L R K A I E E G K L E S F V T D F Y Q R Q G R E V P P L N V D

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P0A847-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P0A847-F1.cif  





Publications:

Title Authors Journal Details PubMed Id DOI
New function of vitamin B12: cobamide-dependent reduction of epoxyqueuosine to queuosine in tRNAs of Escherichia coli and Salmonella typhimurium. Frey B, McCloskey J, Kersten W, Kersten H J Bacteriol [details] 3129401 -

Links:

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