Modomics - A Database of RNA Modifications

ID Card:

Full name: Mitochondrial transcription factor 1
Synonym: MTF1
GI: 1709156
Orf: YMR228W
UniProt: P14908
Structures: | 1I4W |
Alpha Fold Predicted Structure: AF-P14908-F1
Enzyme type: methyltransferase


PDB Structures:


1I4W

Structure Description:

Title: THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION
Classification: TRANSCRIPTION
Technique: X-Ray Diffraction
Resolution: 2.60
R value free: 0.274
R value observed: 0.187
R value work: 0.187

Abstract of the PDB Structure's related Publication:

Although it is commonly accepted that binding of mitochondrial transcription factor sc-mtTFB to the mitochondrial RNA polymerase is required for specific transcription initiation in Saccharomyces cerevisiae, its precise role has remained undefined. In the present work, the crystal structure of sc-mtTFB has been determined to 2.6 A resolution. The protein consists of two domains, an N-terminal alpha/beta-domain and a smaller domain made up of four alpha-helices. Contrary to previous predictions, sc-mtTFB does not resemble Escherichia coli sigma-factors but rather is structurally homologous to rRNA methyltransferase ErmC'. This suggests that sc-mtTFB functions as an RNA-binding protein, an observation standing in contradiction to the existing model, which proposed a direct interaction of sc-mtTFB with the mitochondrial DNA promoter. Based on the structure, we propose that the promoter specificity region is located on the mitochondrial RNA polymerase and that binding of sc-mtTFB indirectly mediates interaction of the core enzyme with the promoter site.

Download RCSB-PDB Structures:

Pdb Files   1I4W.pdb  
Pdbx/mmCIF Files   1I4W.cif  


Protein sequence:

MSVPIPGIKDISKLKFFYGFKYLWNPTVYNKIFDKLDLTKTYKHPEELKVLDLYPGVGIQSAIFYNKYCPRQYSLLEKRSSLYKFLNAKFEGSPLQILKRDPYDWSTYSNLIDEERIFVPEVQSSDHINDKFLTVANVTGEGSEGLIMQWLSCIGNKNWLYRFGKVKMLLWMPSTTARKLLARPGMHSRSKCSVVREAFTDTKLIAISDANELKGFDSQCIEEWDPILFSAAEIWPTKGKPIALVEMDPIDFDFDVDNWDYVTRHLMILKRTPLNTVMDSLGHGGQQYFNSRITDKDLLKKCPIDLTNDEFIYLTKLFMEWPFKPDILMDFVDMYQTEHSG

Comments:

AdoMet is the methyl group donor. Mitochondrial. Numbers of positions like in the bacterial SSU rRNA.





Alpha Fold Predicted Structure:




Downloading... [130810/326360]


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Protein sequence:

M S V P I P G I K D I S K L K F F Y G F K Y L W N P T V Y N K I F D K L D L T K T Y K H P E E L K V L D L Y P G V G I Q S A I F Y N K Y C P R Q Y S L L E K R S S L Y K F L N A K F E G S P L Q I L K R D P Y D W S T Y S N L I D E E R I F V P E V Q S S D H I N D K F L T V A N V T G E G S E G L I M Q W L S C I G N K N W L Y R F G K V K M L L W M P S T T A R K L L A R P G M H S R S K C S V V R E A F T D T K L I A I S D A N E L K G F D S Q C I E E W D P I L F S A A E I W P T K G K P I A L V E M D P I D F D F D V D N W D Y V T R H L M I L K R T P L N T V M D S L G H G G Q Q Y F N S R I T D K D L L K K C P I D L T N D E F I Y L T K L F M E W P F K P D I L M D F V D M Y Q T E H S G
50100150200250300SequenceGHITSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P14908-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P14908-F1.cif  
DSSP Secondary Structures   P14908.dssp  





Publications: