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Find DNAzymes that cleave a DNA/RNA substrate
Enter one sequence in FASTA, EMBL, GenBank or RAW format
(maximum allowed length 2000 nucleotides)
T
U
ACT
AGT
or Upload a FASTA file
Substrate
DNA
RNA (All RNA and Chimeric RNA)
Optional Parameters
Cleave substrate after position
DNAzyme
10-12opt
10-23
10MD5
10MD5-AC
11PC5
13PB2
13PD1
17E
6SE10
6SE11
6SE12
6SE15
6SE20
6SE22
6SE3
6SE30
6YF13
6YF15
6YF16
6YF2
6YF20
6YF27
6YJ10
6YJ14
6YL11
6YL24
6YL34
6YL4
6YR25
7VH6
7VK55
7YE19
7YE2
7YE5
7YE8
7YK24
7YK34
7YK35
7ZG2
7ZG5
7ZJ12
7ZJ7
8-17
8NLJ1
8VA10
8VA11
8VA2
8VA23
8VA25
8VA4
8VA5
8VA6
8VA8
8VB1
8VB12
8VB16
8VB18
8VB2
8VB20
8VB21
8VB22
8VB25
8VB26
8VB4
8VB5
8VB7
8VB9
8VL51
8YG1
8YG24
8YG29
8YG7
8YM17
8YM20
8YM26
8YM4
9NL1
9NL12
9NL27
9NL33
9SE20
9SE22
9SE23
9SE33
9SH18
9SH2
9SH25
9SH3
9SH5
9SK1
9SK17
9SK2
9SK29
9SK31
9SK5
9ZH5
9ZL1
A-2
A-3
A-3(CM1)
A-3(CM2)
A-3(CM3)
A-3(CM4)
A-3(CM5)
AA07
AA14
AA17
AC17
Ag10c
Ce13d
Dz15WS_E
Dz27WS_E
F-8
F-8 (ATP)
F-8 (x)
F-8(CM1)
F-8(CM2)
F-8(CM3)
F-8(CM4)
F-8(CM5)
F-8(CM6)
G5_mini*
HD2
I-R3
II-R1
Lu12
NaA43
NaH1
PLDz
RadDz3
RadDz3(CM1)
RadDz3(CM2)
RadDz6
Tm7
VMA15
VMA8
VMC10
pH
All
< 6
6-7
> 7
Reaction
All
DNA oxidative cleavage
DNA hydrolytic cleavage
DNA cleavage through deglycosylation flowed by two beta elimination
RNA hydrolytic cleavage
NR
RNA transesterification cleavage
Binding arms length
Optimal
Desired length
Desired melting temperature
Left-arm length
Right-arm length
Melting Temperature Left Arm
Melting Temperature Right Arm
Original reported Yield
All
100%
80-100%
60-100%
40-100%
Cofactors
All
Mn2+
Zn2+
Mg2+
Cu2+
Ca2+
Ce3+
Pb2+
Yb3+
Eu3+
Nd3+
Tm3+
Er3+
Na+
Ag+
Histidine
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