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Ethidium bromide intercalation and chromatin structure: a spectropolarimetric analysis

Overview of Vergani L et al.

AuthorsVergani L  Gavazzo P  Mascetti G  Nicolini C  
AffiliationInstitute of Biophysics   Medical School   University of Genoa   Italy.  
JournalBiochemistry
Year 1994

Abstract


Native chromatin has been characterized at different ionic strengths, according to different methods of preparation, by means of circular dichroism and ethidium bromide intercalation. For a more precise interpretation of these results, a series of analogous experiments on phagic DNA, as a function of its circularization, and on mononucleosomes have been performed. Results are discussed here in terms of high order chromatin structure, DNA supercoiling, and topological constraints.