Ethidium bromide intercalation and chromatin structure: a spectropolarimetric analysis
Overview of Vergani L et al.
Authors | Vergani L  Gavazzo P  Mascetti G  Nicolini C   |
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Affiliation | Institute of Biophysics   Medical School   University of Genoa   Italy.   |
Journal | Biochemistry |
Year | 1994 |
Abstract
Native chromatin has been characterized at different ionic strengths, according to different methods of preparation, by means of circular dichroism and ethidium bromide intercalation. For a more precise interpretation of these results, a series of analogous experiments on phagic DNA, as a function of its circularization, and on mononucleosomes have been performed. Results are discussed here in terms of high order chromatin structure, DNA supercoiling, and topological constraints.