Targeting duplex DNA with DNA-PNA chimeras? Physico-chemical characterization of a triplex DNA-PNA/DNA/DNA
Overview of Petraccone L et al.
| Authors | Petraccone L Erra E Messere A Montesarchio D Piccialli G De Napoli L Barone G Giancola C |
|---|---|
| Affiliation | Dipartimento di Chimica Università Federico II di Napoli Via Cintia 4 80126-Napoli Italy. |
| Journal | Biopolymers |
| Year | 2004 |
Abstract
Targeting double-stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA-PNA chimeras. A 16-mer 5'-DNA-3'-p-(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all-DNA complex.