Parallel-stranded linear homoduplexes of d(A+-G)n > 10 and d(A-G)n > 10 manifesting the contrasting ionic strength sensitivities of poly(A+.A+) and DNA
Overview of Dolinnaya NG et al.
| Authors | Dolinnaya NG Ulku A Fresco JR |
|---|---|
| Affiliation | Department of Molecular Biology Princeton University Princeton NJ 08544-1014 USA. |
| Journal | Nucleic Acids Res |
| Year | 1997 |
Abstract
In contrast to shorter homologs which only form a single-stranded nucleic acid alpha-helix in acid solution at [Na+]/=0.2 M) the protonated base-backbone interactions are so weakened that duplex stability becomes increasingly dependent upon H-bonded base pairing and stacking and almost independent of pH. Between pH 6 and 8 this duplex structure is devoid of protonated dA residues and shows positive dependence of T m on ionic strength similar to that of DNA.