Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: differential binding of P and M helical enantiomers
Overview of Meistermann I et al.
| Authors | Meistermann I Moreno V Prieto MJ Moldrheim E Sletten E Khalid S Rodger PM Peberdy JC Isaac CJ Rodger A Hannon MJ |
|---|---|
| Affiliation | Department of Chemistry University of Warwick Coventry CV4 7AL United Kingdom. |
| Journal | Proc Natl Acad Sci U S A |
| Year | 2002 |
Abstract
We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 10(7) M(-1) and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.