A novel pyrimidine tetrad contributing to stabilize tetramolecular G-quadruplex structures
Overview of Esposito V et al.
| Authors | Esposito V Pepe A Filosa R Mayol L Virgilio A Galeone A |
|---|---|
| Affiliation | Department of Pharmacy University of Naples Federico II Via D. Montesano 49 80131 Naples Italy. galeone@unina.it antonella.virgilio@unina.it. |
| Journal | Org Biomol Chem |
| Year | 2016 |
Abstract
G-quadruplex structures formed by oligodeoxyribonucleotides TGGU(NH2)GGT (AM, U(NH2) = 5-amino-2'-deoxyuridine), TGGU(Br)GGT (BR, U(Br) = 5-bromo-2'-deoxyuridine) and TGGTGGT (TH) have been investigated through circular dichroism, nuclear magnetic resonance, gel electrophoresis and molecular modeling techniques. Collected data indicate that all 7-mer oligonucleotides form tetramolecular parallel G-quadruplex structures with all residues adopting anti glycosidic bonds. In the case of AM, data suggest the occurrence of a novel U(NH2)-tetrad characterized by eight hydrogen bonds that stabilizes the G-quadruplex structure more efficiently than U(Br)- and T-tetrads.