Study on the adsorption of DNA on the layered double hydroxides (LDHs)
Overview of Li B et al.
| Authors | Li B Wu P Ruan B Liu P Zhu N |
|---|---|
| Affiliation | College of Environment and Energy South China University of Technology Guangzhou 510006 PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters Ministry of Education Guangzhou 510006 PR China. |
| Journal | Spectrochim Acta A Mol Biomol Spectrosc |
| Year | 2013 |
Abstract
Four kinds of layered double hydroxides (LDHs) were prepared by chemical coprecipitation method and used as DNA adsorbents. Multiple characterization tools such as power X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and Standard electronic modules (SEM) were employed to characterize the LDHs. By examining the effect of initial concentration, solution pH, adsorption experiments were carried out to investigate the adsorption capacities of LDHs for DNA. The results revealed that the LDHs with Mg/Al=3 had higher ability on adsorbing the DNA and were not affected by pH values. The LDHs exhibited excellent adsorption properties and completely adsorbed DNA within 2h. The adsorption equilibrium data were fitted to the Langmuir and Freundlich models, showing that the Langmuir model which represented monolayer adsorption had better correlation with the adsorption linear equation. In addition, Circular dichroism (CD) spectrum, UV-vis spectorscopy and agarose gel electrophoresis revealed the integrity of DNA structure, suggesting that there had no damage on the DNA structure during the adsorption process.