Modomics - A Database of RNA Modifications

ID Card:

Full name: Cytoplasmic tRNA 2-thiolation protein 1
Synonym: Ctu1, Tuc1
GI: 50593215
Orf: YGL211W
COG: COG0037
UniProt: P53088
Alpha Fold Predicted Structure: AF-P53088-F1
Complex: Nsc2/Ncs6
Enzyme type: thiolase
Position of modification - modification: t:34 - cmnm5s2U



Protein sequence:

MSFTAPSDPVNKPTKVKVSQLCELCHSRKALIRRPKNLSKLCKQCFCLVFETEIHNTIVANNLFQRGEKVAVGASGGKDSTVLAHMLKLLNDRYDYGIEIVLLSIDEGIIGYRDDSLATVKRNQQQYGLPLEIFSFKDLYDWTMDEIVSVAGIRNSCTYCGVFRRQSLDRGAAKLGISHVVTGHNADDMAETVLMNILRGDVARLEKSTAIITQSSGSPIKRSKPFKYSYQKEIVLYAHYMKLDYFSTECTYAPEAFRGTAREYMKNLEAVRPSCIIDIIQSGENLALKAKKSNAGKRVVKFVDGNRCARCGYLSSNNICKACMLLEGLEKSRAQVAIENDTSADGAALKLRALEKLSF

Comments:

Both Ncs6 and Ncs2 are necessary for the thiolation of cytoplasmic tRNA. For more information see the entry for yeast Nsc2. High sequence similarities exist between bacterial eukaryal Ncs6 (Tuc1) and bacterial TtcA responsible for the thiolation of C32 in anticodon loop of tRNA (convergent evolution). They might have evolved from a common thiolase precursor. Ncs2 and Ncs6 are down-regulated in a proteasomal dependent fashion in yeast exposed to mild heat stress. This down-regulation influences translation of mRNAs rich in codons decoded by tRNAs thiolated by the Ncs2-Ncs6 complex.





Alpha Fold Predicted Structure:




Parsing response... [326569/326569]


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Protein sequence:

M S F T A P S D P V N K P T K V K V S Q L C E L C H S R K A L I R R P K N L S K L C K Q C F C L V F E T E I H N T I V A N N L F Q R G E K V A V G A S G G K D S T V L A H M L K L L N D R Y D Y G I E I V L L S I D E G I I G Y R D D S L A T V K R N Q Q Q Y G L P L E I F S F K D L Y D W T M D E I V S V A G I R N S C T Y C G V F R R Q S L D R G A A K L G I S H V V T G H N A D D M A E T V L M N I L R G D V A R L E K S T A I I T Q S S G S P I K R S K P F K Y S Y Q K E I V L Y A H Y M K L D Y F S T E C T Y A P E A F R G T A R E Y M K N L E A V R P S C I I D I I Q S G E N L A L K A K K S N A G K R V V K F V D G N R C A R C G Y L S S N N I C K A C M L L E G L E K S R A Q V A I E N D T S A D G A A L K L R A L E K L S F
50100150200250300350SequenceGHTBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P53088-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P53088-F1.cif  
DSSP Secondary Structures   P53088.dssp  





Publications:

Links:

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