Modomics - A Database of RNA Modifications

ID Card:

Full name: Probable inactive tRNA-specific adenosine deaminase-like protein 3
Synonym: TAD3
GI: 74731634
UniProt: Q96EY9
Alpha Fold Predicted Structure: AF-Q96EY9-F1
Enzyme type: deaminase



Protein sequence:

MEPAPGLVEQPKCLEAGSPEPEPAPWQALPVLSEKQSGDVELVLAYAAPVLDKRQTSRLLKEVSALHPLPAQPHLKRVRPSRDAGSPHALEMLLCLAGPASGPRSLAELLPRPAVDPRGLGQPFLVPVPARPPLTRGQFEEARAHWPTSFHEDKQVTSALAGRLFSTQERAAMQSHMERAVWAARRAAARGLRAVGAVVVDPASDRVLATGHDCSCADNPLLHAVMVCVDLVARGQGRGTYDFRPFPACSFAPAAAPQAVRAGAVRKLDADEDGLPYLCTGYDLYVTREPCAMCAMALVHARILRVFYGAPSPDGALGTRFRIHARPDLNHRFQVFRGVLEEQCRWLDPDT

Comments:

Multiple mRNA codons can be decoded by a single tRNA via the post-transcriptional modification of tRNA wobble adenosine. In eukaryotes, adenosine-to-inosine modification is catalyzed by ADAR enzyme family (Savva et al. 2012 ). ADAT is a heterodimeric enzyme belonging to this family that acts on tRNAs. Evolutionary models exploring the sequence homology of ADAT and ADARs suggest these tRNA modifying enzymes be ancestral to ADARs enzymes. ADAT is composed of two subunits named ADAT2 and ADAT3. ADAT2 is the catalytic subunit of the ADAT complex. However, several ADAT3 residues are required for the ADAT complex to be functionally active (Ramos-Morales et al. 2021). Mutations in this enzyme cause different diseases, principally related to neurological disorders. ADAT complex modifies up to eight tRNA transcripts in their 34 anticodon adenosine. (Ramos-Morales et al. 2021)




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
A:I tRNA (t)   34 AGT IGT tRNAThrAGT anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AGC IGC tRNAAlaAGC anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AGG IGG tRNAPro AGG anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AGA IGA tRNASerAGA anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AAG IAG tRNALeuAGG anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AAT IAT tRNAIleAAT anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 AAC IIC tRNAValAAC anticodon-loop Nucleoplasm 30590541   
A:I tRNA (t)   34 ACG ICG tRNAArgACG anticodon-loop Nucleoplasm 30590541   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M E P A P G L V E Q P K C L E A G S P E P E P A P W Q A L P V L S E K Q S G D V E L V L A Y A A P V L D K R Q T S R L L K E V S A L H P L P A Q P H L K R V R P S R D A G S P H A L E M L L C L A G P A S G P R S L A E L L P R P A V D P R G L G Q P F L V P V P A R P P L T R G Q F E E A R A H W P T S F H E D K Q V T S A L A G R L F S T Q E R A A M Q S H M E R A V W A A R R A A A R G L R A V G A V V V D P A S D R V L A T G H D C S C A D N P L L H A V M V C V D L V A R G Q G R G T Y D F R P F P A C S F A P A A A P Q A V R A G A V R K L D A D E D G L P Y L C T G Y D L Y V T R E P C A M C A M A L V H A R I L R V F Y G A P S P D G A L G T R F R I H A R P D L N H R F Q V F R G V L E E Q C R W L D P D T

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q96EY9-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q96EY9-F1.cif  
DSSP Secondary Structures   Q96EY9.dssp  





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