Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA-queuine glycosyltransferase
Structures: | |
Enzyme type: hexose transferase
Position of modification - modification: t:34 - manQ
t:34 - galQ

Protein sequence:


Hexose-transferase (Htr). Utilizes GDP-mannose, GDP-galactose In tRNAAsp (anticodon QUC) and tRNATyr (anticodon QYA) from certain eukaryotic cells (e.g. mammalian hepatocytes), the nucleoside Q-34 is further hypermodified into a glycosylated derivative by tRNA-queuine glycosyltransferase. This enzymatic activity was detected in rat liver extract and Xenopus leavis oocytes but the protein was not purified.

Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
Q:galQ tRNA (t) Tyr/GUA/prokaryotic cytosol 34
Q:manQ tRNA (t) Asp/GUC/prokaryotic cytosol 34


Title Authors Journal Details PubMed Id DOI
The RNA Modification Database: 1999 update. Rozenski J, Crain PF, McCloskey JA Nucleic Acids Res [details] 9847178 -
Enzymatic formation of queuosine and of glycosyl queuosine in yeast tRNAs microinjected into Xenopus laevis oocytes. The effect of the anticodon loop sequence. Haumont E, Droogmans L, Grosjean H Eur J Biochem [details] 3117541 -
Structure, biosynthesis, and function of queuosine in transfer RNA. Nishimura S Prog Nucleic Acid Res Mol Biol [details] 6410456 -
Enzymatic synthesis of Q nucleoside containing mannose in the anticodon of tRNA: isolation of a novel mannosyltransferase from a cell-free extract of rat liver. Okada N, Nishimura S Nucleic Acids Res [details] 20603 -