Modomics - A Database of RNA Modifications

ID Card:

Full name: Mitochondrial translation optimization protein 1
Synonym: Ips1
GI: 115502421
Orf: YGL236C
COG: COG0445
UniProt: P53070
Alpha Fold Predicted Structure: AF-P53070-F1
Complex: Mto1/Mss1
Enzyme type: other
Position of modification - modification: t:34 - cmnm5s2U



Protein sequence:

MLRVTTLASSCTSFPLQVLRRRLTISSLTSFQPTTKTQVVVIGAGHAGCEAAAASSRTGAHTTLITPSLTDIGKCSCNPSIGGVGKGILVKEIDALDGLMGKVTDLAGVQFKMLNRSKGPAVWGPRAQIDRELYKKYMQRELSDKKAHPNLSLLQNKVADLILYDPGCGHKVIKGVVLDDGTQVGADQVIITTGTFLSAEIHIGDKRIAAGRIGEQPTYGISNTLQNEVGFQLGRLKTGTPARLAKESIDFSALEVQKGDALPVPMSFLNETVSVEPTKQLDCFGTHTTPQMHDFLRNNLHQSIHIQDTTIKGPRYCPSIEAKILRFPDRSSHKIWLEPEGFNSDVIYPNGISNSMPEDVQLQMMRLIPGMANVEILQPAYGVEYDYVDPRQLKPSLETKLVDGLFLAGQINGTTGYEEAAAQGIIAGINAGLLSRQEREQLVLKRSEAYIGVLIDDLINNGVIEPYRMFTSRSEFRISVRADNADFRLTPIGAQLGIISPVRLSQYSRDKHLYDETIRALQNFKLSSQKWSSLLQANIAPQAENRSAWEIFRFKDMDLHKLYECIPDLPINLLDIPMHVVTKINIQGKYEPYIVKQNQFVKAFQADENMLLPQDYDYRQLPTLSTECKLLLNRVQPLTIGQARRIQGITAAALFELYRVARKPSQPVM

Comments:

Yeast mitochondrial homolog of bacterial MnmG (GidA). Favoprotein. Makes a stable complex with Mss1p. Does not work on cytoplasmic tRNAs. Belongs to the bacterial gidA family but is distinct from bacterial Gid, renamed TrmFO.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
s2U:cmnm5s2U RNA tRNA 34 2UU $UU tRNALeu2AA wobble - position Mitochondrion 15509579   
U:cmnm5U RNA tRNA 34 UUU !UU tRNALysUUU wobble - position Mitochondrion

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M L R V T T L A S S C T S F P L Q V L R R R L T I S S L T S F Q P T T K T Q V V V I G A G H A G C E A A A A S S R T G A H T T L I T P S L T D I G K C S C N P S I G G V G K G I L V K E I D A L D G L M G K V T D L A G V Q F K M L N R S K G P A V W G P R A Q I D R E L Y K K Y M Q R E L S D K K A H P N L S L L Q N K V A D L I L Y D P G C G H K V I K G V V L D D G T Q V G A D Q V I I T T G T F L S A E I H I G D K R I A A G R I G E Q P T Y G I S N T L Q N E V G F Q L G R L K T G T P A R L A K E S I D F S A L E V Q K G D A L P V P M S F L N E T V S V E P T K Q L D C F G T H T T P Q M H D F L R N N L H Q S I H I Q D T T I K G P R Y C P S I E A K I L R F P D R S S H K I W L E P E G F N S D V I Y P N G I S N S M P E D V Q L Q M M R L I P G M A N V E I L Q P A Y G V E Y D Y V D P R Q L K P S L E T K L V D G L F L A G Q I N G T T G Y E E A A A Q G I I A G I N A G L L S R Q E R E Q L V L K R S E A Y I G V L I D D L I N N G V I E P Y R M F T S R S E F R I S V R A D N A D F R L T P I G A Q L G I I S P V R L S Q Y S R D K H L Y D E T I R A L Q N F K L S S Q K W S S L L Q A N I A P Q A E N R S A W E I F R F K D M D L H K L Y E C I P D L P I N L L D I P M H V V T K I N I Q G K Y E P Y I V K Q N Q F V K A F Q A D E N M L L P Q D Y D Y R Q L P T L S T E C K L L L N R V Q P L T I G Q A R R I Q G I T A A A L F E L Y R V A R K P S Q P V M

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P53070-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P53070-F1.cif  
DSSP Secondary Structures   P53070.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae. Colby G, Wu M, Tzagoloff A J Biol Chem [details] 9774408 -
Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases. Umeda N, Suzuki T, Yukawa M, Ohya Y, Shindo H, Watanabe K, Suzuki T J Biol Chem [details] 15509579 -

Links:

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