Abstract:
In vitro selection experiments yield unexpected results. An RNA‐cleaving DNA enzyme that was selected in the presence of Mg2+ ions is much more efficient with Ca2+ as the cofactor. The result is even more surprising because of the lower hydrolytic efficiency of Ca2+ itself.
DNAzymes linked to this article:
| Name | Isolated sequence | Length | Reaction |
|---|---|---|---|
| class 6 | TAGGAAGTAGGGACCTACAAGTTGTCATTGTAACTGAGTTCTGCCAGCTGsACGAAATAGTCAGGAGTTAG | 71 | RNA cleavage |
| class 1 | AAGCTCTCTCAGCGAGACGAAATAGGGAGTTAGCAGCACGAGGTTACACTTTTATCCTCTCCCAAAGTAGGGAC | 74 | RNA cleavage |
| class 3 | GGCATGTACCCAAGAAGGGGTGGAGCCGGCAGTGACCCCTGCGAGTAGGGAAGCCCAAGAACGAAGAGTTAAGC | 74 | RNA cleavage |
| class 4 | ATGCTGTTGCTCTGTCAGCGGACACGAAATAGTGGGTCGCGATGCTGAATAATCACGGCGAAAGTGGTTGCCTA | 74 | RNA cleavage |
| class 5 | CAGATGTGAAGTTAGAGCTTTGCCAGCnTCGTTAGTAGAGTCAGCGCACAGGGGAAGATTGTATGTCTATAG | 72 | RNA cleavage |
| class 7 | CGTCATGCGAAAAGAATGGTGAGATTTGCCAGCsGTCGAGTAGTAGTCCAGGGAACATTGCCCGGGGGATT | 71 | RNA cleavage |
| class 8 | AGAACGGTAGAGTGCNGGGGGTGCAGTTGAGCTTTGTCAGCsACACGAATAAGAGTCTCGTAGGATTCACCAAG | 74 | RNA cleavage |
| Mg5 (class 2) | AGCACGCATGACTTTGATGCCACGTAAAGTGAAAGAGCTGTTGATCTGTCAGCGACACGAAATGGTGATGCCCT | 74 | RNA cleavage |