DNAmoreDB - A Database of Deoxyribozymes

Abstract:

In vitro selection of RNA-cleaving DNAzymes is a powerful method for isolating metal-specific DNA. A few successful examples are known, but it is still difficult to target some thiophilic metals such as Cd2+ due to limited functional groups in DNA. While using modified bases expands the chemical functionality of DNA, a single phosphorothioate modification might boost its affinity for thiophilic metals without complicating the selection process or using bases that are not commercially available. In this work, the first such in vitro selection for Cd2+ is reported. After using a blocking DNA and negative selections to rationally direct the library outcome, a highly specific DNAzyme with only 12 nucleotides in the catalytic loop is isolated. This DNAzyme has a cleavage rate of 0.12 min−1 with 10 μM Cd2+ at pH 6.0. The Rp form of the substrate is cleaved ∼100-fold faster than the Sp form. The DNAzyme is most active with Cd2+ and its selectivity against Zn2+ is over 100 000-fold. Its application in detecting Cd2+ is also demonstrated. The idea of introducing single modifications in the fixed region expands the scope of DNA/metal interactions with minimal perturbation of DNA structure and property.

DNAzymes linked to this article:

Name	Isolated sequence	Length	Reaction
BN-Cd35	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTGCATAAATGTCTACATGCAGAATATCCGCCATTTCATTCGACAGTAGAGATAGTGACGGTAAGCTT	114	RNA cleavage
UNBlkCd07	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTGGGAGCCATAGGTCAAAGGTGGGTGCGGTCGTTTATCGACTAGTTATAATGACGGTAAGCTTGG	111	RNA cleavage
UNBlkCd34	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTGGGAGCCATAGGTCAAAGGTGGGTGCGTGTCGTATATCGACTAAGTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd14	CTGCAGAATTCTAATACGAGTCACTATAGGAAGTTGGCGAAACATTTCGAGCCATAGGTCAAAGGTGGGTGCGGGTCGTATATCGACTAAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd04	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTTGGTGCTGGTCGCATTATATCGACTAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd08	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCATGGAGCCATAGGTCAAAGGTGGGTGCTGTCGCATCATATCGACTGTTATAGTAACGGTAAGCTTGG	113	RNA cleavage
UNBlkCd23	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCTAGTCGTATCATATCGACTAGTATAGTGACGGTAAGCTTGG	111	RNA cleavage
UNBlkCd30	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCTAGTCGTATCATATCGACTAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
Ni03b	GTCGCCAAACTCAAGAAGAGGACCAGGATAGTGAGGT	37	RNA cleavage
UNBlkCd22	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATTGAGCCATAGGTCAAAGGTGGGTGCTAGTCGTATCATACCGACTAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd05	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAGTATAGTGACGGTAAGCTTGG	111	RNA cleavage
UNBlkCd21	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAGTATAGTGACGGTAAGCTTGG	111	RNA cleavage
UNBlkCd03	CTGCAGAATTCTAATACGAGTCACTATAAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAGTTATAGTGACGGTAAGCTTGG	113	RNA cleavage
UNBlkCd36	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd15	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd11	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd10	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd09	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd06	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd17	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATACCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd37	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATACCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd40	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGGCGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd35	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCAGAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd02	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCGAAGGTGGGCGCGAGTCGTATCATATCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd38	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCACATCGACGAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd26	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAAGTTATAGTGACGGTAAGCTTGG	113	RNA cleavage
UNBlkCd25	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAAGTTATAGTGACGGTAAGCTTGG	113	RNA cleavage
UNBlkCd19	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGAATCATGTCGACGAAGTTATAGTGACGGTAAGCTTGG	113	RNA cleavage
UNBlkCd12	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCTTATCGACGAAGTTATAGTGGCGGTAAGCTTGG	113	RNA cleavage
UNBlkCd28	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCTTATCGACGAAGTTATAGTGACGGTAAGCTTGG	113	RNA cleavage
UNBlkCd27	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGTGGTCGTATCTTATCGACCAGTAATAGTGACGGTAAGCTTGG	111	RNA cleavage
UNBlkCd29	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCGACGAAGTAATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd33	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGAAGCCATAGGTCAAAGGTGGGTGCGAGTCGTATCATATCAACTCGTATAGTGACGGTAAGCTTGG	111	RNA cleavage
UNBlkCd13	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGTGTCGTATCATATCGACTAAGTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd24	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATGGAGCCATAGGTCAAAGGTGGGTGCGTGTCGATCTAATCGACTAAGTTATAGTGACGGTAAGCTTGG	112	RNA cleavage
UNBlkCd20	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACAAAAAAACTCAAAAGTTGGCCCTTCCCGCATTTAAGTTTTCGGAAGATAGTGACGGTAAGCTTGG	116	RNA cleavage
UNBlkCd39	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAAGGAGCAAATAGCGAGAAACCGGCGAAACTAGAGGCGATCTGGGTTAGTGACGGTAAGCTTGG	116	RNA cleavage
BlkCd07	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTCATTCGATAGTTGAAATAGGTACAAGTATCACGGTGATATTGTATCATGTTAGTGTCG	106	RNA cleavage
BlkCd26	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTCATTCGATAGTTGAAATAGGTACGAGTATCACGGTGATATTGTATCATGTTAGTGACG	107	RNA cleavage
BlkCd03	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTATCACGCGATAGAAATAGCGACAAGTCTAGGTGTGATTTATGCTCTCTTCTAGTGACG	106	RNA cleavage
BlkCd10	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTAAGTCATCTAATCACTCGAAGAAGAGTGGCGAGGAGTAAGAATGTCGTGATGGTGACG	106	RNA cleavage
BlkCd31	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTCACTAGTAAAGCAAAGCATAGAGCTCTACGGGTTAGGGGTACGAGGTCGTTGGTGACG	106	RNA cleavage
BlkCd18	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTAGTCAGAATACGGACAAAGAGTGGCAGACAGAAACCTTCGATAGCTCAAATAGTGACG	107	RNA cleavage
BlkCd23	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATTTTTAGAGCATAAAACCAAAATTTGTTAAGACAGTGACCTTCGATAGCACAAATAGTGACG	106	RNA cleavage
BN-Cd12	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTGCATAAATGTCTACATGCAGAATATCCACCATTTCATTCGACAGTAGAGATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd09	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTAGACGGCACCTGAGATGATTTAATCGCAGTTCTTCCTTCGATAGCTAAGATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd34	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTAGACGGCACCTGAGATGATTTAATCGCAGTTCTTCCTTCGATAGCTAAGATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd24	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTAGACGGCACCTGAGCTGATATAATCGCACTTCTTCCTTCGATAGCTAAGATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd25	CTGCAGAATTCTAATACSAGTCACTATAGGAAGATGGCGAAACATCTTAAACCAGGTGTCTTACTTTCTAAGCTGTCATCTTCATTCGATAGCACAAATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd36	CTGCAGAATTCTAATACSAGTCACTATAGGAAGATGGCGAAACATCTTATGAGCTAACGTAAAAGTTTCTAAAGCCACTGTTCCTTCSATAGTACAGATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd17	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTTTATTAAAACGTGTAAAAATGTGGGGGCAGTTTCCTTCGATAGCCCAGATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd23	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTTTATTAAAACGTGTAAAAATGTAGGGGCAGTTTCCTTCGATAGCCCAGATAGTGACGGTAAGCTT	114	RNA cleavage
BlkCd19	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTTAGATACTTATAAATGGTCAATGAGCAATGTTCAGTAGCTTGTGCGACTTAGTGACG	106	RNA cleavage
BlkCd16	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACGTCCACTGATAGAGCTCATATTGGAAAGGAATATTGGTTATGAACCCTAGTTACG	106	RNA cleavage
BlkCd01	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAGGCATTGAAATACTTTTGGATTTATATTATCATATGGCCGGGAGATAGTGACG	106	RNA cleavage
BlkCd27	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAGCCTGTGCCGCATCGTTAGGATTACGATAAGAACACCGCGGGAAATAGTGACG	106	RNA cleavage
BlkCd15	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAGCACGTAATGCCTCATGCTCTGTATAATGAGGGGAACGTGAGGGTTAGTGACG	106	RNA cleavage
BlkCd21	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAACGGTAGCACGCCGAAAGTGCTATACAGAGGGGAGTAGTGTGGGTTAGTGACG	106	RNA cleavage
BlkCd02	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTACGGAAACTACGTCGCGTGCATTTAAAAGGCGAATAGAGAGGGGGTTAGTGACG	106	RNA cleavage
BlkCd22	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTAATGGTTTACATAGTCCATCTAAGACCTTGTCACCTTCATCGCGAAGAAATAGTGACG	106	RNA cleavage
BlkCd30	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAGGAGCTAGGCCTCCTTATATTTTATCACGAGTAGCTCACGGGAGATAGTGACG	106	RNA cleavage
BlkCd09	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAAGGCTCAATGAGCCGCGAAGGCGGCATGCATACAAGTCTGGGAGATAGTGACG	106	RNA cleavage
BlkCd24	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAAGGATAGTTAGTTGTAGGTTGACAACGTGTGGGACTAGACTGGGTTAGTGACG	106	RNA cleavage
BlkCd36	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAATGTGTAGGAGACTCCCATCGTTATGGACATGTCTCTACATGGGGTAGTCACG	106	RNA cleavage
BlkCd06	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAATAAATGGAGTGGTCAACCGAATTAGGAGCACGTAATGCATGAGTTAGTGACG	106	RNA cleavage
BlkCd11	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAGCAAGTTTATTGCGGGTTAATGACAGTTATAAAGCGTATCATTACTAGTGACG	106	RNA cleavage
BlkCd14	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTGCTGAAACATTGTTCAGTAGCCTTTTATTAACACTAATAAAGAGCGACTTAGTGACG	106	RNA cleavage
BlkCd39	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACAAGAAAGCATATTCGCAGCAGGGGTATAGCGAAGATGAGTCGGAAGATAGTGACCG	107	RNA cleavage
BlkCd12	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAACTAACGGAAAGCAAGTTGAAGAATCGATTGTCGATTGTGGGAGATAGTAACG	106	RNA cleavage
BlkCd38	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTAGTAACAAACACACTAGGGTAAATATTGGGATTTCGAGTGGTTGCGCCTTAGTGACG	106	RNA cleavage
BlkCd33	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTATCCGACGAGGAGCCCTGGGCCAGGGGGATATCAGTTGGAGGGAGATAGTGACG	106	RNA cleavage
BlkCd40	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAGTAAAAAGCCCAGCTATGGGTAGGTGTGGGTATCCGTTTACGGGATAGTGACG	106	RNA cleavage
BlkCd20	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTTAGTAGCACATCAGGAGAAACGTGGAAAAGATAGCTACTGAATGGCGGCTTAGTGACG	106	RNA cleavage
BlkCd25	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAACAATCTGTATTATTGCTGTGGGGTGAGTTACAGAAGGTGGGAGGTAGTGACG	106	RNA cleavage
BlkCd32	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAAGCAATAGCAAAGGATTGCAGAATTTTCTATGGCTATCGCTGGGTTAGTGACG	106	RNA cleavage
BlkCd35	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACTAAGCAATAGCAAAGGATTGCAGAATTTTCTATGGCTATCGCTGGGTTAGTGACG	106	RNA cleavage
BlkCd28	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTTACTAGTTTAACAATGACACCAAAGAATGGAGGGACATTGTTTCGCGGGGTAGTGACG	106	RNA cleavage
BlkCd05	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACAAGATCAGGGGCGATAAGTCATAGAACAAAGGATCTCCTGATCGGGTTAGTGACG	106	RNA cleavage
BlkCd17	CTGCAGAATTCTAATACGAGTCACTATAGGAAATGGCGAAACATCTTTAGTAGTTGTACTTAACCGGGGACAGAAGACATAGAGGGTATCAACGCCTTAGTGACG	105	RNA cleavage
BlkCd04	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTAGTAGTTTAAACCGGAGTTGTCAATCAGACGTATGAAGGAAAAACGCCTTAGTGACG	106	RNA cleavage
BlkCd13	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTAGTTATAAACCGGATTCACTAATAGGCTGATACAGAGAGGGCTATGGGTTAGTGACG	106	RNA cleavage
BlkCd08	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTAGTAGTTCCAAGGGGATAAAAATGGAAGAGCGGGCCCATGGGAACGCCTTAGTGACG	106	RNA cleavage
BlkCd34	CTGCAGAATTCGCCCTTGGAAGATGGCGAAACATCTTTAGTAATGGTCTCCGACGTCTAAACTGGGTCGGACTAGAAGTTAATGCCTTAGTGACG	95	RNA cleavage
BN-Cd02	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTAGAGATCTATTGAACGATAACTAATTAGCCATATTTATCCACCTACATCTTAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd20	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTAGCCAGCTGAAACAATCGAAGAGTTTTGCATATCGTGATGACGCAAAGAGTAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd01	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTAATCGGTAACAGCAACAATAATAGGTTTCTACTGCTACGTAGGGCCAATTAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd10	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTAATCGGTAACAGCAACAATAATAGGTTTCTACTGCTACGTAGGGCCAATTAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd06	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTTAATCGGTAACAGCGACAATAATAAGTTTGTACTGCTACGTAGGGCCAATTAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd30	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTAATCGGTAACAGCAACAATAATAAGTTTGTACTGCTACGTAGGGCCAATTAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd13	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTGCGTATCATCTTCAATTCGATAGAGTCCACGTCTACAGGAATGTGGGAAATAGTAACGGTAAGCTT	114	RNA cleavage
BN-Cd14	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTGCGTATCATCTTCAATTCGATAGAGTCCACGTCTACAGGAATGTGGGAAATAGTAACGGTAAGCTT	114	RNA cleavage
BN-Cd38	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATATTGCTCATYATCYTCAATTCGATAGAGTCCACGTTCACAAGAATGTGGGAAATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd04	CTGCAGAATTCTAATACGAGTCACTATAGGAGATGGCGAAACATTTAGGGGTCCATATTGCTCAAAGATAGTTCGAACATCTGAAACGCACGAAGAATAGTGACGGTAAGCTT	113	RNA cleavage
BN-Cd21	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTGGGGTCTATATTGCTCAAAGATAGTTCGTACAACTGAAACGCACGAAGAATAATGACGGTAAGCTT	114	RNA cleavage
BN-Cd40	CTGCAGAATTCTAATAGAGTCACTATAGGAAGATGGCGAAACATCTTGGGGCACGCTCTTAACCAAGATAATGTTAAGTATCTTACAGGAACCACTTTAGTGACGGTAAGCTT	113	RNA cleavage
BN-Cd07	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTGCATAAATGTCTACATGCAGAATATCCGCCATTTCATTCGACAGTAGAGATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd11	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTTTATTTAAACGTGTAAAAATGTAGGGGCATCTTCCTTCGACAGCCCAGATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd03	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTACACTCGAAGAGTCTCTTAAATTATACCTTGTAGAATCCCCTGGAGGAAATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd37	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTACACTCGAAGAGTCTCTTAAATTGTACCCGGTACAATCCCCTGGAGGAAATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd05	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACAATCTTCATTCGATAGTTGAAATAGGTACGAGTATCACGGCGATGTTGTATCATGTTAGTAACGGTAAGCTT	115	RNA cleavage
BN-Cd08	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTCATTCGATAGTTGAAATAGGTACAAGTATCACGGTGATATTGTATCACGTTAGTAACGGTAAGCTT	114	RNA cleavage
BN-Cd15	CTGCAGAATTCTAATAACGAGTCACTATAGGAAGATGGCGAAACATCTTCATTCGATAGTTGAGATAGGTACGAGTATCACGGTCATATTGTATCATGTTGGTGACGGTAAGCTT	115	RNA cleavage
BN-Cd29	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTCATTCGATAGTTGAGATAGGTACAAGTATCACGGTGACATTGTATCATGTTAGTGTCGGTAAGCTT	114	RNA cleavage
BN-Cd19	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTCATTCGATAGTTAAAATAGGTACGAGTATCACGGTGATATTGTACCATGTTAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd33	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTCATTCGATAGTTAAAATTGGGAGGACATGTAGTGGGACGATTCAACCCCCTAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd26	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTCATTCGATAGCTCAAATAGGACTATGTGTTTAACTGGAATATGAAAGGACTAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd16	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTCTTCGATAGTTAAAATAGTGACTTCTATATTAAGTCGCCTCATTGTTGATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd22	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTCTTCGATAGTTAAGATAGTGACTTCTATATTAAGTCGTCTCATTGTTAATAATGACGGTAAGCTT	114	RNA cleavage
BN-Cd32	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACCCAAAAGGAGTTCTTACGGATCCTACAATGAGGAAAAGTATGAGTTATAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd39	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACACCTTTAATCCAAACCACGGTGGTGACTGGCCTGAATAAGAGCATTAGACTATATTAGTGACGGTAAGCTT	114	RNA cleavage
BN-Cd18	CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACCCAAAAGGAAGGTTTTCTAATAACTGGCTTTAGTAGAAACACAGGAGTAGTGACGGTAAGCTT	114	RNA cleavage

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