DNAmoreDB - A Database of Deoxyribozymes

Abstract:

The mercury project: A self‐cleaving DNAzyme that “switches on” in the presence of Hg2+ has been revealed by in vitro selection. The enzyme 10‐13 (see picture) utilizes two modified nucleobases to confer an RNaseA‐like catalytic activity that requires only Hg2+ and no other divalent metal cofactor. This DNAzyme is highly selective for mercuric cations.

DNAzymes linked to this article:

Name	Isolated sequence	Length	Reaction
10-3	CACAGUGUGUGAGGCACUGUACGGUGAGUGGUGCUU	36	RNA cleavage
10-5	UUCUCAUCCGUAGUGAGGGACGCGGCGCUCCCCCGUU	37	RNA cleavage
10-8	CAUAGUGCGUGAGGCCCGCCCACUCCCCGUCGUGGUA	37	RNA cleavage
10-9	UUCUCAUCCGUAGUGAGGGACGCGGCGCUCCCCCGUU	37	RNA cleavage
10-10	GCACAGUGUGUGAGGCAUGUGCGAGUGUGCUGUCUCG	37	RNA cleavage
10-13	CACACGUGUGUGACGGCCUCGCGCGCCCGCCCUGCCGUA	39	RNA cleavage
10-17	CAUAGUGCGUGAGGCGCGUCUGCAGCGUGGUGGGUUU	37	RNA cleavage
10-21	CACAGUUGUGUGAUGGCUGGGCAGCCAGCGGUGGUCA	37	RNA cleavage
10-24	CAUAGUGCGUGAGGCUUGCGCUGUCAGCGGUCG	33	RNA cleavage
10-31	CACAGUUGUGUGAGGCGCGUGUACAGUGCGGCGGGUCU	38	RNA cleavage
10-34	CACAGUUGUGUGAUGGCUGGCUCUGCAGCCCGCUGGGU	38	RNA cleavage
10-40	CACACGUGUGUGAGGCAUGCGCUCACUGCCGUGGGUCU	38	RNA cleavage
10-41	CACAGUGUGUGAGGUUCGCCUCUGCCUCCCUGCUACA	37	RNA cleavage
10-42	CAUAGUGCGUGAGGCACGGUUACCGCCGUGGUGUGU	36	RNA cleavage
10-49	CACAGUGUGUGAGGCGUGCGCGAGUGGUUAGUGUCCUG	38	RNA cleavage

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