| Reaction | Reacting groups | Substrates | Product | Metal ion | Seq description | Covalent Modification of Phosphorylated Amino Acid Side Chains |
Group 1 - Phosphoserine |
L: DNA-anchored AAAXAA hexapeptide X: X = phosphoserine (SP) |
Dehydroalanine |
Mn2+ Mg2+ Zn2+ |
N 40 |
|---|
| Buffer conditions |
|---|
| 70 mM HEPES pH 7.5, 1 mM ZnCl2, 20 mM MnCl2, 40 mM MgCl2, 150 mM NaCl |
| Yield (%) | kcat/ kobs |
|---|---|
| 68 | kobs = 0.43 h-1 |
| Catalytic region of the DNAzyme |
|---|
| ACAGGATTCAAAGTGGCTGTCAGAAGTTGGTGAGGGAAG |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2015 | J Chandrasekar | S K Silverman | Phosphoserine Lyase Deoxyribozymes: DNA-Catalyzed Formation of Dehydroalanine Residues in Peptides. | 26200899 | 10.1021/jacs.5b06308 | Covalent Modification of Phosphorylated Amino Acid Side Chains |