| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | Ester hydrolysis |
Group 1 - H2O |
S: Ester |
Carboxylic acid |
Mg2+ |
ester | N 40 |
|---|
| Buffer conditions |
|---|
| 50 mM CHES pH 9.0, 40 mM MgCl2,150 mM NaCl |
| Yield (%) | kcat/ kobs |
|---|---|
| 70-80 | kobs = 1.7 h-1 |
| Catalytic region of the DNAzyme |
|---|
| GGGGGCGCGTCATCATCGAAAGGGGGTCCTGCATTACGCC |
| Notes |
|---|
| The ester substrate is covalently attached to a DNA anchor oligonucleotide. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2013 | B M Brandsen | S K Silverman | DNA-catalyzed hydrolysis of esters and aromatic amides. | 24127695 | 10.1021/ja4077233 | Ester hydrolysis |