Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of G15 Group 2 - vicinal phosphate |
S: AUAGACUGAAUGAAGGACUUCCGUAACU |
cleavage at G15 |
Mg2+ |
RNA phosphodiester | N 20 |
---|
Buffer conditions |
---|
500 mM Tris-HCl, 1.5 M NaCl, 5mM MgCl2 |
Yield (%) | kcat/ kobs |
---|---|
42 | kobs = 0.0011 min-1 |
Catalytic region of the DNAzyme |
---|
GGGTTCGGTGGAGCGGCGCGA |
Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
---|---|---|---|---|---|---|
2020 | A Liaqat | C Höbartner | N 6-isopentenyladenosine in RNA determines the cleavage site of endonuclease deoxyribozymes. | 32681686 | 10.1002/anie.202006218 | RNA cleavage |