| Reaction | Substrates | Product | Metal ion | Seq description | Porphyrin metalation |
S: mesoporphyrin IX (MPIX) |
Cu-MPIX or Zn-MPIX |
Pb2+ |
N * |
|---|
| Buffer conditions |
|---|
| 100 mM Tris-HCl pH 7.5, 200 mM NaOAc, 25 mM KOAc, 10 mM Mg(OAc)2 |
| kcat/ kobs |
|---|
| kcat = 0.89 min-1 |
| Catalytic region of the DNAzyme |
|---|
| GGGTGGGTGGGTGGGT |
| Notes |
|---|
| The reported buffer was used to study the Cu<sup>2+</sup> insertion kinetics into MPIX. The authors report that T30695 was not obtained from via SELEX, but was identified by screening a small set of DNA sequences. The related publication (by Yang H. et al, 2020) reports newly discovered properties of T30695, such as the insertion of Zn<sub>2+</sub> in other porphyrins, and the utilization of Pb<sub>2+</sub> as a substrate. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2019 | D Peng | J Liu | Efficient DNA-Catalyzed Porphyrin Metalation for Fluorescent Ratiometric Pb Detection. | 31414597 | 10.1021/acs.analchem.9b02759 | Porphyrin metalation |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2020 | H Yang | J Liu | Pb2+ as a Substrate and a Cofactor of a Porphyrin Metalation DNAzyme. | 32202058 | 10.1002/cbic.202000073 |
Porphyrin metalation |